AFLP comparisons among Claviceps africana isolates from the United States, Mexico, Africa, Australia, India, and Japan

Paul W. Tooley, Erin D Goley, Marie M. Carras, Nichole R. O'Neill

Research output: Contribution to journalArticle

Abstract

Eighty-seven isolates of the sorghum ergot pathogen, Claviceps africana, from diverse geographic locations were analyzed using four different amplified fragment length polymorphism (AFLP) primer combinations to determine genetic relationships among isolates. Most isolates showed unique AFLP haplotypes, indicating that substantial genetic variation is present within C. africana populations. Two major groupings of isolates were observable, with ca. 70% similarity between the two groups. One group consisted of Australian, Indian, and Japanese isolates and the other of U.S., Mexican, and African isolates. In spite of overall high levels of genetic diversity observed in C. africana, isolates within the two major groups were between 75 and 100% similar. The observed associations of C. africana isolates from worldwide sources could be the result of intercontinental trade and/or movement of seed. The data indicate that Africa was the likely source of C. africana that has become established in the Americas since 1996. Analysis of additional isolates in future studies will reveal whether these groupings are being maintained or whether population subdivision or reshuffiing may occur.

Original languageEnglish (US)
Pages (from-to)1247-1252
Number of pages6
JournalPlant Disease
Volume86
Issue number11
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Claviceps
amplified fragment length polymorphism
Mexico
Japan
ergot
India
genetic variation
founder effect
genetic relationships
haplotypes
pathogens
seeds

Keywords

  • Population genetics
  • Sphacelia sorghi
  • Sugary disease

ASJC Scopus subject areas

  • Plant Science

Cite this

AFLP comparisons among Claviceps africana isolates from the United States, Mexico, Africa, Australia, India, and Japan. / Tooley, Paul W.; Goley, Erin D; Carras, Marie M.; O'Neill, Nichole R.

In: Plant Disease, Vol. 86, No. 11, 01.11.2002, p. 1247-1252.

Research output: Contribution to journalArticle

Tooley, Paul W. ; Goley, Erin D ; Carras, Marie M. ; O'Neill, Nichole R. / AFLP comparisons among Claviceps africana isolates from the United States, Mexico, Africa, Australia, India, and Japan. In: Plant Disease. 2002 ; Vol. 86, No. 11. pp. 1247-1252.
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