TY - JOUR
T1 - Aflatoxins isolated by immunoaffinity chromatography from foods consumed in the Gambia, West Africa
AU - Hudson, Geoffrey J.
AU - Wild, Christopher P.
AU - Zarba, Audrey
AU - Groopman, John D.
PY - 1992
Y1 - 1992
N2 - An aflatoxin‐specific, monoclonal antibody‐based immunoaffinity chromatography method has been developed for the rapid isolation of aflatoxins from human foods. Aflatoxins were isolated by immunoaffinity chromatography from a variety of cooked foods, including maize, rice, millets, groundnut sauces, and leaf sauces, collected in The Gambia, West Africa. The aflatoxins were measured by direct fluorescence or high‐pressure liquid chromatography. The highest levels were found for groundnut sauces, mean 162 ppb (range 18 to 943 ppb) for 18 positive samples, but aflatoxins were found in other foods; e.g., maize, mean 9.7 ppb (range 2 to 35 ppb) for nine positive samples. The food analysis results were used with records of the amounts of cooked food to estimate a mean daily intake for an individual of the order of 3.5 μg of aflatoxins per day. This approach for exposure assessment is considered in relation to other biomarkers of aflatoxin exposure using biological fluids. © 1992 Wiley‐Liss, Inc.
AB - An aflatoxin‐specific, monoclonal antibody‐based immunoaffinity chromatography method has been developed for the rapid isolation of aflatoxins from human foods. Aflatoxins were isolated by immunoaffinity chromatography from a variety of cooked foods, including maize, rice, millets, groundnut sauces, and leaf sauces, collected in The Gambia, West Africa. The aflatoxins were measured by direct fluorescence or high‐pressure liquid chromatography. The highest levels were found for groundnut sauces, mean 162 ppb (range 18 to 943 ppb) for 18 positive samples, but aflatoxins were found in other foods; e.g., maize, mean 9.7 ppb (range 2 to 35 ppb) for nine positive samples. The food analysis results were used with records of the amounts of cooked food to estimate a mean daily intake for an individual of the order of 3.5 μg of aflatoxins per day. This approach for exposure assessment is considered in relation to other biomarkers of aflatoxin exposure using biological fluids. © 1992 Wiley‐Liss, Inc.
KW - Chemical carcinogens
KW - Liver cancer
KW - Mycotoxins
UR - http://www.scopus.com/inward/record.url?scp=0027028554&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027028554&partnerID=8YFLogxK
U2 - 10.1002/nt.2620010208
DO - 10.1002/nt.2620010208
M3 - Article
C2 - 1344903
AN - SCOPUS:0027028554
SN - 1056-9014
VL - 1
SP - 100
EP - 105
JO - Natural toxins
JF - Natural toxins
IS - 2
ER -