TY - JOUR
T1 - Aflatoxin, a human carcinogen
T2 - determination in foods and biological samples by monoclonal antibody affinity chromatography.
AU - Groopman, J. D.
AU - Donahue, K. F.
PY - 1988
Y1 - 1988
N2 - We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.
AB - We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.
UR - http://www.scopus.com/inward/record.url?scp=0024084070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024084070&partnerID=8YFLogxK
U2 - 10.1093/jaoac/71.5.861
DO - 10.1093/jaoac/71.5.861
M3 - Article
C2 - 3235405
AN - SCOPUS:0024084070
SN - 0004-5756
VL - 71
SP - 861
EP - 867
JO - Journal - Association of Official Analytical Chemists
JF - Journal - Association of Official Analytical Chemists
IS - 5
ER -