Aflatoxin, a human carcinogen: determination in foods and biological samples by monoclonal antibody affinity chromatography.

John Davis Groopman, K. F. Donahue

Research output: Contribution to journalArticle

Abstract

We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.

Original languageEnglish (US)
Pages (from-to)861-867
Number of pages7
JournalJournal of the Association of Official Analytical Chemists
Volume71
Issue number5
StatePublished - Sep 1988
Externally publishedYes

Fingerprint

Affinity chromatography
Antibody Affinity
Aflatoxins
carcinogen
carcinogens
affinity chromatography
Affinity Chromatography
aflatoxins
Carcinogens
antibody
chromatography
monoclonal antibodies
Monoclonal Antibodies
Food
food
Aflatoxin M1
Environmental Carcinogens
Technology
aflatoxin M1
sampling

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

@article{13de813b44ba489fb80ec7f0f538dbc1,
title = "Aflatoxin, a human carcinogen: determination in foods and biological samples by monoclonal antibody affinity chromatography.",
abstract = "We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.",
author = "Groopman, {John Davis} and Donahue, {K. F.}",
year = "1988",
month = "9",
language = "English (US)",
volume = "71",
pages = "861--867",
journal = "Journal of AOAC International",
issn = "1060-3271",
publisher = "AOAC International",
number = "5",

}

TY - JOUR

T1 - Aflatoxin, a human carcinogen

T2 - determination in foods and biological samples by monoclonal antibody affinity chromatography.

AU - Groopman, John Davis

AU - Donahue, K. F.

PY - 1988/9

Y1 - 1988/9

N2 - We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.

AB - We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.

UR - http://www.scopus.com/inward/record.url?scp=0024084070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024084070&partnerID=8YFLogxK

M3 - Article

C2 - 3235405

AN - SCOPUS:0024084070

VL - 71

SP - 861

EP - 867

JO - Journal of AOAC International

JF - Journal of AOAC International

SN - 1060-3271

IS - 5

ER -