Aflatoxin, a human carcinogen: determination in foods and biological samples by monoclonal antibody affinity chromatography.

J. D. Groopman, K. F. Donahue

Research output: Contribution to journalArticle

Abstract

We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.

Original languageEnglish (US)
Pages (from-to)861-867
Number of pages7
JournalJournal - Association of Official Analytical Chemists
Volume71
Issue number5
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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