Affordable, abbreviated roche monitor assay for quantification of human immunodeficiency virus type 1 RNA in plasma

Susan A. Fiscus, Shu Chen, Donald Hoover, Melissa G. Kerkau, Paul Alabanza, Som Siharath, John Schmitz, Newton Kumwenda, Taha E. Taha

Research output: Contribution to journalArticle

Abstract

The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.

Original languageEnglish (US)
Pages (from-to)4200-4202
Number of pages3
JournalJournal of clinical microbiology
Volume43
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Microbiology (medical)

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