Affinity chromatography of 3-oxosteroid Δ4-Δ5-isomerase of Pseudomonas testosteroni

Ann M. Benson, Anthony J. Suruda, Roger Shaw, Paul Talalay

Research output: Contribution to journalArticle

Abstract

Affinity chromatography of 3-oxosteroid Δ45-isomerase (EC 5.3.3.1) of Pseudomonas testosteroni on 19-nortestosterone-17-O-succinyl diaminodipropylamino-agarose constitues a powerful and highly effective step in the purification of the enzyme in preparation for crystallization.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume348
Issue number2
DOIs
StatePublished - May 29 1974

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steroid delta-isomerase
Comamonas testosteroni
Ketosteroids
Nandrolone
Affinity chromatography
Isomerases
Crystallization
Affinity Chromatography
Sepharose
Purification
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology
  • Medicine(all)

Cite this

Affinity chromatography of 3-oxosteroid Δ4-Δ5-isomerase of Pseudomonas testosteroni. / Benson, Ann M.; Suruda, Anthony J.; Shaw, Roger; Talalay, Paul.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 348, No. 2, 29.05.1974, p. 317-320.

Research output: Contribution to journalArticle

Benson, Ann M. ; Suruda, Anthony J. ; Shaw, Roger ; Talalay, Paul. / Affinity chromatography of 3-oxosteroid Δ4-Δ5-isomerase of Pseudomonas testosteroni. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1974 ; Vol. 348, No. 2. pp. 317-320.
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