Aerosolization of infectious virus by excimer laser

L. B. Moreira; D. Sanchez; M. D. Trousdale; D. Stevenson; F. Yarber; P. J. McDonnell

doi:10.1016/S0002-9394(14)70124-2

Aerosolization of infectious virus by excimer laser

L. B. Moreira, D. Sanchez, M. D. Trousdale, D. Stevenson, F. Yarber, P. J. McDonnell

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

PURPOSE: To determine the potential for aerosolization of infectious virus present within the tear film during excimer laser photoablation of the cornea. METHODS: Cell monolayers infected with herpes simplex virus or adenovirus, simulating virus-infected corneas, were ablated with the 193-nm excimer laser. Adjacent dishes containing noninfected cell monolayers were subsequently assayed for viral infection. RESULTS: Viral spread to sentinel dishes occurred with both herpes simplex and adenovirus. The titer of virus present in the infected cell monolayers influenced the likelihood of spread to adjacent dishes. The presence of a vacuum aspiration system appeared to influence the direction of virus spread, with dishes located in the direction of the vacuum most likely to contain virus. CONCLUSIONS: The potential for aerosolization of infectious virus exists with photoablation using a large- diameter excimer laser beam. Our experimental design, however, does not prove that spread of infectious virus is likely to occur in the clinical setting. Appropriate measures should be taken to reduce the possibility of the spread of virus from the patient to the surgeon, other medical staff, or other patients.

Original language	English (US)
Pages (from-to)	297-302
Number of pages	6
Journal	American journal of ophthalmology
Volume	123
Issue number	3
DOIs	https://doi.org/10.1016/S0002-9394(14)70124-2
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology

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10.1016/S0002-9394(14)70124-2

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title = "Aerosolization of infectious virus by excimer laser",

abstract = "PURPOSE: To determine the potential for aerosolization of infectious virus present within the tear film during excimer laser photoablation of the cornea. METHODS: Cell monolayers infected with herpes simplex virus or adenovirus, simulating virus-infected corneas, were ablated with the 193-nm excimer laser. Adjacent dishes containing noninfected cell monolayers were subsequently assayed for viral infection. RESULTS: Viral spread to sentinel dishes occurred with both herpes simplex and adenovirus. The titer of virus present in the infected cell monolayers influenced the likelihood of spread to adjacent dishes. The presence of a vacuum aspiration system appeared to influence the direction of virus spread, with dishes located in the direction of the vacuum most likely to contain virus. CONCLUSIONS: The potential for aerosolization of infectious virus exists with photoablation using a large- diameter excimer laser beam. Our experimental design, however, does not prove that spread of infectious virus is likely to occur in the clinical setting. Appropriate measures should be taken to reduce the possibility of the spread of virus from the patient to the surgeon, other medical staff, or other patients.",

author = "Moreira, {L. B.} and D. Sanchez and Trousdale, {M. D.} and D. Stevenson and F. Yarber and McDonnell, {P. J.}",

note = "Funding Information: Accepted for publication Oct 16, 1996. From the Doheny Eye Institute and the Department of Ophthalmology, University of Southern California School of Medicine, Los Angeles, California. This study was supported in part by National Eye Institute/ National Institutes of Health core grants EY03040 and EY09417 and by an unrestricted grant from Research to Prevent Blindness, Inc, New York, New York. Presented in part at the Eye Bank Association of America annual meeting, June 29, 1996, Santa Barbara, California. Correspondence to Peter J. McDonnell, MD, Doheny Eye Institute, 1450 San Pablo St, Los Angeles, CA 90033. No reprints are available.",

year = "1997",

doi = "10.1016/S0002-9394(14)70124-2",

language = "English (US)",

volume = "123",

pages = "297--302",

journal = "American journal of ophthalmology",

issn = "0002-9394",

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TY - JOUR

T1 - Aerosolization of infectious virus by excimer laser

AU - Moreira, L. B.

AU - Sanchez, D.

AU - Trousdale, M. D.

AU - Stevenson, D.

AU - Yarber, F.

AU - McDonnell, P. J.

N1 - Funding Information: Accepted for publication Oct 16, 1996. From the Doheny Eye Institute and the Department of Ophthalmology, University of Southern California School of Medicine, Los Angeles, California. This study was supported in part by National Eye Institute/ National Institutes of Health core grants EY03040 and EY09417 and by an unrestricted grant from Research to Prevent Blindness, Inc, New York, New York. Presented in part at the Eye Bank Association of America annual meeting, June 29, 1996, Santa Barbara, California. Correspondence to Peter J. McDonnell, MD, Doheny Eye Institute, 1450 San Pablo St, Los Angeles, CA 90033. No reprints are available.

PY - 1997

Y1 - 1997

N2 - PURPOSE: To determine the potential for aerosolization of infectious virus present within the tear film during excimer laser photoablation of the cornea. METHODS: Cell monolayers infected with herpes simplex virus or adenovirus, simulating virus-infected corneas, were ablated with the 193-nm excimer laser. Adjacent dishes containing noninfected cell monolayers were subsequently assayed for viral infection. RESULTS: Viral spread to sentinel dishes occurred with both herpes simplex and adenovirus. The titer of virus present in the infected cell monolayers influenced the likelihood of spread to adjacent dishes. The presence of a vacuum aspiration system appeared to influence the direction of virus spread, with dishes located in the direction of the vacuum most likely to contain virus. CONCLUSIONS: The potential for aerosolization of infectious virus exists with photoablation using a large- diameter excimer laser beam. Our experimental design, however, does not prove that spread of infectious virus is likely to occur in the clinical setting. Appropriate measures should be taken to reduce the possibility of the spread of virus from the patient to the surgeon, other medical staff, or other patients.

AB - PURPOSE: To determine the potential for aerosolization of infectious virus present within the tear film during excimer laser photoablation of the cornea. METHODS: Cell monolayers infected with herpes simplex virus or adenovirus, simulating virus-infected corneas, were ablated with the 193-nm excimer laser. Adjacent dishes containing noninfected cell monolayers were subsequently assayed for viral infection. RESULTS: Viral spread to sentinel dishes occurred with both herpes simplex and adenovirus. The titer of virus present in the infected cell monolayers influenced the likelihood of spread to adjacent dishes. The presence of a vacuum aspiration system appeared to influence the direction of virus spread, with dishes located in the direction of the vacuum most likely to contain virus. CONCLUSIONS: The potential for aerosolization of infectious virus exists with photoablation using a large- diameter excimer laser beam. Our experimental design, however, does not prove that spread of infectious virus is likely to occur in the clinical setting. Appropriate measures should be taken to reduce the possibility of the spread of virus from the patient to the surgeon, other medical staff, or other patients.

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Aerosolization of infectious virus by excimer laser

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