AEG-1 promoter-mediated imaging of prostate cancer

Akrita Bhatnagar, Yuchuan Wang, Ronnie C. Mease, Matthew Gabrielson, Polina Sysa, Il Minn, Gilbert Green, Brian Simmons, Kathleen Gabrielson, Siddik Sarkar, Paul B. Fisher, Martin G. Pomper

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Abstract

We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [18F]fluorodeoxyglucose and [18F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.

Original languageEnglish (US)
Pages (from-to)5772-5781
Number of pages10
JournalCancer Research
Volume74
Issue number20
DOIs
StatePublished - Oct 15 2014

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Bhatnagar, A., Wang, Y., Mease, R. C., Gabrielson, M., Sysa, P., Minn, I., Green, G., Simmons, B., Gabrielson, K., Sarkar, S., Fisher, P. B., & Pomper, M. G. (2014). AEG-1 promoter-mediated imaging of prostate cancer. Cancer Research, 74(20), 5772-5781. https://doi.org/10.1158/0008-5472.CAN-14-0018