AEG-1 promoter-mediated imaging of prostate cancer

Akrita Bhatnagar; Yuchuan Wang; Ronnie C. Mease; Matthew Gabrielson; Polina Sysa; Il Minn; Gilbert Green; Brian Simmons; Kathleen Gabrielson; Siddik Sarkar; Paul B. Fisher; Martin G. Pomper

doi:10.1158/0008-5472.CAN-14-0018

AEG-1 promoter-mediated imaging of prostate cancer

Akrita Bhatnagar, Yuchuan Wang, Ronnie C. Mease, Matthew Gabrielson, Polina Sysa, Il Minn, Gilbert Green, Brian Simmons, Kathleen Gabrielson, Siddik Sarkar, Paul B. Fisher, Martin G. Pomper

School of Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [¹⁸F]fluorodeoxyglucose and [¹⁸F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.

Original language	English (US)
Pages (from-to)	5772-5781
Number of pages	10
Journal	Cancer Research
Volume	74
Issue number	20
DOIs	https://doi.org/10.1158/0008-5472.CAN-14-0018
State	Published - Oct 15 2014

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1158/0008-5472.CAN-14-0018

Cite this

@article{ad602be797c24b948ec71cf1b6705845,

title = "AEG-1 promoter-mediated imaging of prostate cancer",

abstract = "We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [18F]fluorodeoxyglucose and [18F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.",

author = "Akrita Bhatnagar and Yuchuan Wang and Mease, {Ronnie C.} and Matthew Gabrielson and Polina Sysa and Il Minn and Gilbert Green and Brian Simmons and Kathleen Gabrielson and Siddik Sarkar and Fisher, {Paul B.} and Pomper, {Martin G.}",

note = "Publisher Copyright: {\textcopyright}2014 AACR.",

year = "2014",

month = oct,

day = "15",

doi = "10.1158/0008-5472.CAN-14-0018",

language = "English (US)",

volume = "74",

pages = "5772--5781",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

number = "20",

}

TY - JOUR

T1 - AEG-1 promoter-mediated imaging of prostate cancer

AU - Bhatnagar, Akrita

AU - Wang, Yuchuan

AU - Mease, Ronnie C.

AU - Gabrielson, Matthew

AU - Sysa, Polina

AU - Minn, Il

AU - Green, Gilbert

AU - Simmons, Brian

AU - Gabrielson, Kathleen

AU - Sarkar, Siddik

AU - Fisher, Paul B.

AU - Pomper, Martin G.

PY - 2014/10/15

Y1 - 2014/10/15

N2 - We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [18F]fluorodeoxyglucose and [18F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.

AB - We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [18F]fluorodeoxyglucose and [18F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.

UR - http://www.scopus.com/inward/record.url?scp=84908146948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908146948&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-14-0018

DO - 10.1158/0008-5472.CAN-14-0018

M3 - Article

C2 - 25145668

AN - SCOPUS:84908146948

SN - 0008-5472

VL - 74

SP - 5772

EP - 5781

JO - Cancer Research

JF - Cancer Research

IS - 20

ER -

AEG-1 promoter-mediated imaging of prostate cancer

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this