TY - JOUR
T1 - Advantages of cryopreserved lymphocytes for sequential evaluation of human immune competence. II. Mixed lymphocyte cultures and mononuclear cell subpopulations
AU - Sears, Henry F.
AU - Tondreau, Susan C.
AU - Rosenberg, Steven A.
PY - 1978/10
Y1 - 1978/10
N2 - Peripheral blood lymphocytes were obtained from normal volunteer donors over a 2-week period. One-way or twoway mixed lymphocyte reactions, percentages of T-cells and B- cells, and percentages of monocytes were quantitated on both fresh and cryopreserved lymphocytes. These comparisons revealed that the measurement of mixed leukocyte reactions, fractions of E-rosettes and EA rosettes, and the fraction of esterase-staining cells were not altered by cryopreservation and storage at -196° C up to 1 year. Cryopreserved reference lymphocytes provided a stable standard for use as stimulating cells in mixed lymphocyte cultures and could be irradiated or treated with mitomycin C prior to being frozen, without alteration of their function as stimulator cells. Cryopreserved cells thus appeared to be utilizable in the sequential measurement of the immune competence of humans.
AB - Peripheral blood lymphocytes were obtained from normal volunteer donors over a 2-week period. One-way or twoway mixed lymphocyte reactions, percentages of T-cells and B- cells, and percentages of monocytes were quantitated on both fresh and cryopreserved lymphocytes. These comparisons revealed that the measurement of mixed leukocyte reactions, fractions of E-rosettes and EA rosettes, and the fraction of esterase-staining cells were not altered by cryopreservation and storage at -196° C up to 1 year. Cryopreserved reference lymphocytes provided a stable standard for use as stimulating cells in mixed lymphocyte cultures and could be irradiated or treated with mitomycin C prior to being frozen, without alteration of their function as stimulator cells. Cryopreserved cells thus appeared to be utilizable in the sequential measurement of the immune competence of humans.
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U2 - 10.1093/jnci/61.4.1011
DO - 10.1093/jnci/61.4.1011
M3 - Article
C2 - 151750
AN - SCOPUS:0018131530
SN - 0027-8874
VL - 61
SP - 1011
EP - 1016
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -