Advantages of cryopreserved lymphocytes for sequential evaluation of human immune competence. II. Mixed lymphocyte cultures and mononuclear cell subpopulations

Henry F. Sears, Susan C. Tondreau, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

Peripheral blood lymphocytes were obtained from normal volunteer donors over a 2-week period. One-way or twoway mixed lymphocyte reactions, percentages of T-cells and B- cells, and percentages of monocytes were quantitated on both fresh and cryopreserved lymphocytes. These comparisons revealed that the measurement of mixed leukocyte reactions, fractions of E-rosettes and EA rosettes, and the fraction of esterase-staining cells were not altered by cryopreservation and storage at -196° C up to 1 year. Cryopreserved reference lymphocytes provided a stable standard for use as stimulating cells in mixed lymphocyte cultures and could be irradiated or treated with mitomycin C prior to being frozen, without alteration of their function as stimulator cells. Cryopreserved cells thus appeared to be utilizable in the sequential measurement of the immune competence of humans.

Original languageEnglish (US)
Pages (from-to)1011-1016
Number of pages6
JournalJournal of the National Cancer Institute
Volume61
Issue number4
DOIs
StatePublished - 1978
Externally publishedYes

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Mental Competency
Cell Culture Techniques
Lymphocytes
Mixed Lymphocyte Culture Test
Cryopreservation
Mitomycin
Esterases
Monocytes
Healthy Volunteers
B-Lymphocytes
Tissue Donors
Staining and Labeling
T-Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Advantages of cryopreserved lymphocytes for sequential evaluation of human immune competence. II. Mixed lymphocyte cultures and mononuclear cell subpopulations. / Sears, Henry F.; Tondreau, Susan C.; Rosenberg, Steven A.

In: Journal of the National Cancer Institute, Vol. 61, No. 4, 1978, p. 1011-1016.

Research output: Contribution to journalArticle

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