Advancing signaling networks through proteomics

Tina L. Tekirian, Stefani N. Thomas, Austin Yang

Research output: Contribution to journalReview article

Abstract

Healthful physiology can be distinguished from unhealthful physiology by focusing upon how a given signal transduction pathway is shifted as a function of disease. In order to distinguish between pathways that contribute to normal versus disease biology, it is necessary to identify components that comprise a protein module. The development of methods that target such differences is essential for the identification, development and validation of biomarkers that can improve the quality of diagnoses and treatment of disease. This review discusses the use of proteomic methods that integrate cell biology, mass spectrometry and bioinformatics, in relation to the analyses of protein signaling modules that are subject to differential phosphorylation. We examine how these methods can be used to distinguish abnormal from normal physiology.

Original languageEnglish (US)
Pages (from-to)573-583
Number of pages11
JournalExpert Review of Proteomics
Volume4
Issue number4
DOIs
StatePublished - Aug 1 2007

Keywords

  • Affinity
  • Crosslinking
  • Phosphoproteomics
  • Protein signaling module
  • Purification
  • Quantitative mass spectrometry
  • Systems reconstruction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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