Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve

Peggi M. Angel, H. Scott Baldwin, Danielle Gottlieb Sen, Yan Ru Su, John E. Mayer, David Bichell, Richard R. Drake

Research output: Contribution to journalReview articlepeer-review

Abstract

Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve. Protein imaging by MALDI IMS allows multiplexed histological mapping of proteins and protein components that are inaccessible by antibodies and should be considered an important tool for basic and clinical cardiovascular research. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.

Original languageEnglish (US)
Pages (from-to)927-935
Number of pages9
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1865
Issue number7
DOIs
StatePublished - Jul 2017
Externally publishedYes

Keywords

  • Cardiac
  • Cardiovascular
  • Extracellular matrix
  • Imaging mass spectrometry
  • Protein imaging
  • Semilunar valve

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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