Advanced fluorescence imaging endoscopy using an acousto-optic tuneable filter

Maurice P. Whelan, Mounir Bouhifd, Marc Aprahamian

Research output: Contribution to journalConference articlepeer-review

Abstract

Two novel prototype instruments for in vivo fluorescence-based medical diagnostics are described. The devices are based on an acousto-optic tuneable filter (AOTF) and can be easily attached to the eyepiece of most commercially available endoscopes. The instruments developed offer significant advantages over typical fixed-filter or filter-wheel fluorescence imaging systems in terms of flexibility, performance and diagnostic potential. Any filtering centre-wavelength in the range from 450 to 700nm can be rapidly selected either by random access or sequential tuning using simple commands delivered over a PC serial interface. In addition, both filtered and unfiltered light can be imaged to facilitate the direct association of fluorescence signals with specific anatomical sites. To demonstrate the system in vivo, a study of the diagnostic potential of fluorescence imaging for pancreatitis was conducted on rats. The aim was to detect extremely low-levels of endogenous protoporphyrin IX (PpIX) that has been shown to accumulate in early-stage diseased tissue undergoing an inflammatory response. Results show clearly that the device is effective in diagnosing mild pancreatitis in rats without the necessity of administering PpIX promoting agents such as ALA. Planning of human clinical trials is currently underway to demonstrate its potential as a tool for non-invasive early diagnosis of gastroenterological diseases.

Original languageEnglish (US)
Article number5486-45
Pages (from-to)295-306
Number of pages12
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5
Issue number30
DOIs
StatePublished - Dec 1 2004
EventALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics - Silsoe, United Kingdom
Duration: Sep 19 2003Sep 23 2003

Keywords

  • Acousto-optic tuneable filter
  • Endogenous protoporphyrin IX
  • Endoscope
  • In vivo fluorescence imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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