Adult mouse myometrial label-retaining cells divide in response to gonadotropin stimulation

Paul P. Szotek, Henry L. Chang, Li Hua Zhang, Frederic Preffer, David Dombkowski, Patricia K. Donahoe, Jose Teixeira

Research output: Contribution to journalArticlepeer-review

Abstract

Conditional deletion of β-catenin in the Müllerian duct mesenchyme results in a degenerative uterus characterized by replacement of the myometrial smooth muscle with adipose tissue. We hypothesized that the mouse myometrium houses somatic smooth muscle progenitor cells that are hormonally responsive and necessary for remodeling and regeneration during estrous cycling and pregnancy. We surmise that the phenotype observed in β-catenin conditionally deleted mice is the result of dysregulation of these progenitor cells. The objective of this study was to identify the mouse myometrial smooth muscle progenitor cell and its niche, define the surface marker phenotype, and show a functional response of these cells to normal myometrial cycling. Uteri were labeled with 5-bromo-2′-deoxyuridine (BrdU) and chased for up to 14 weeks. Myometrial label-retaining cells (LRCs) were observed in the myometrium and stroma throughout the chase period. After 12 weeks, phenotypic analysis of the LRCs by immunofluorescence demonstrated that the majority of LRCs colocalized with α-smooth muscle actin, estrogen receptor-α, and β-catenin. Flow cytometry of myometrial cells identified a myometrial Hoechst 33342 effluxing "side population" that expresses MISRII-Cre-driven YFP. Functional response of LRCs was investigated by human chorionic gonadotropin stimulation of week 12 chase mice and demonstrated sequential proliferation of LRCs in the endometrial stroma, followed by the myometrium. These results suggest that conventional myometrial regeneration and repair is executed by hormonally responsive stem or progenitor cells derived from the Müllerian duct mesenchyme.

Original languageEnglish (US)
Pages (from-to)1317-1325
Number of pages9
JournalStem Cells
Volume25
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Keywords

  • Label-retaining cells
  • Mesenchymal stem cell
  • Mouse myometrium
  • Myometrial stem cell
  • Stem cell niche

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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