Adult behavior in male mice exposed to E-cigarette nicotine vapors during late prenatal and early postnatal life

Dani Smith, Angela Aherrera, Armando Lopez, Enid Neptune, Jonathan P. Winickoff, Jonathan D. Klein, Gang Chen, Philip Lazarus, Michael Collaco, Sharon A McGrath-Morrow

Research output: Contribution to journalArticle

Abstract

Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. Methods Timed-pregnant C57BL/6J mice were exposed to 2.4% nicotine in propylene glycol (PG) or 0% nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. Results Adult male mice exposed to 2.4% nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0% nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4% nicotine/PG mice spent more than 25% of time in the new location whereas the other groups did not. Conclusion Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth.

Original languageEnglish (US)
Article numbere0137953
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 15 2015

Fingerprint

cigarettes
nicotine
Nicotine
vapors
Tobacco Products
Vapors
Propylene Glycol
propylene glycol
mice
testing
Electronic Cigarettes
Cotinine
Attention Deficit Disorder with Hyperactivity
Inbred C57BL Mouse
Brain
rearing
Head
pregnancy
brain
Pregnancy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Adult behavior in male mice exposed to E-cigarette nicotine vapors during late prenatal and early postnatal life. / Smith, Dani; Aherrera, Angela; Lopez, Armando; Neptune, Enid; Winickoff, Jonathan P.; Klein, Jonathan D.; Chen, Gang; Lazarus, Philip; Collaco, Michael; McGrath-Morrow, Sharon A.

In: PLoS One, Vol. 10, No. 9, e0137953, 15.09.2015.

Research output: Contribution to journalArticle

Smith, Dani ; Aherrera, Angela ; Lopez, Armando ; Neptune, Enid ; Winickoff, Jonathan P. ; Klein, Jonathan D. ; Chen, Gang ; Lazarus, Philip ; Collaco, Michael ; McGrath-Morrow, Sharon A. / Adult behavior in male mice exposed to E-cigarette nicotine vapors during late prenatal and early postnatal life. In: PLoS One. 2015 ; Vol. 10, No. 9.
@article{52cea0ae69644a378099ea4fd036b0b4,
title = "Adult behavior in male mice exposed to E-cigarette nicotine vapors during late prenatal and early postnatal life",
abstract = "Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. Methods Timed-pregnant C57BL/6J mice were exposed to 2.4{\%} nicotine in propylene glycol (PG) or 0{\%} nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. Results Adult male mice exposed to 2.4{\%} nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0{\%} nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4{\%} nicotine/PG mice spent more than 25{\%} of time in the new location whereas the other groups did not. Conclusion Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth.",
author = "Dani Smith and Angela Aherrera and Armando Lopez and Enid Neptune and Winickoff, {Jonathan P.} and Klein, {Jonathan D.} and Gang Chen and Philip Lazarus and Michael Collaco and McGrath-Morrow, {Sharon A}",
year = "2015",
month = "9",
day = "15",
doi = "10.1371/journal.pone.0137953",
language = "English (US)",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Adult behavior in male mice exposed to E-cigarette nicotine vapors during late prenatal and early postnatal life

AU - Smith, Dani

AU - Aherrera, Angela

AU - Lopez, Armando

AU - Neptune, Enid

AU - Winickoff, Jonathan P.

AU - Klein, Jonathan D.

AU - Chen, Gang

AU - Lazarus, Philip

AU - Collaco, Michael

AU - McGrath-Morrow, Sharon A

PY - 2015/9/15

Y1 - 2015/9/15

N2 - Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. Methods Timed-pregnant C57BL/6J mice were exposed to 2.4% nicotine in propylene glycol (PG) or 0% nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. Results Adult male mice exposed to 2.4% nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0% nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4% nicotine/PG mice spent more than 25% of time in the new location whereas the other groups did not. Conclusion Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth.

AB - Nicotine exposure has been associated with an increased likelihood of developing attention deficit hyperactivity disorder (ADHD) in offspring of mothers who smoked during pregnancy. The goal of this study was to determine if exposure to E-cigarette nicotine vapors during late prenatal and early postnatal life altered behavior in adult mice. Methods Timed-pregnant C57BL/6J mice were exposed to 2.4% nicotine in propylene glycol (PG) or 0% nicotine /PG once a day from gestational day 15 until delivery. After delivery, offspring and mothers were exposed to E-cigarette vapors for an additional 14 days from postnatal day 2 through 16. Following their last exposure serum cotinine levels were measured in female juvenile mice. Male mice underwent behavioral testing at 14 weeks of age to assess sensorimotor, affective, and cognitive functional domains. Results Adult male mice exposed to 2.4% nicotine/PG E-cigarette vapors had significantly more head dips in the zero maze test and higher levels of rearing activity in the open field test compared to 0% nicotine/PG exposed mice and untreated controls. In the water maze test after reversal training, the 2.4% nicotine/PG mice spent more than 25% of time in the new location whereas the other groups did not. Conclusion Adult male mice exhibited increased levels of activity in the zero maze and open field tests when exposed to E-cigarette vapor containing nicotine during late prenatal and early postnatal life. These findings indicate that nicotine exposure from E-cigarettes may cause persistent behavioral changes when exposure occurs during a period of rapid brain growth.

UR - http://www.scopus.com/inward/record.url?scp=84945561378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945561378&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0137953

DO - 10.1371/journal.pone.0137953

M3 - Article

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e0137953

ER -