ADPriboDB: The database of ADP-ribosylated proteins

Christina A. Vivelo, Ricky Wat, Charul Agrawal, Hui Yi Tee, Anthony Kar Lun Leung

Research output: Contribution to journalArticle

Abstract

ADP-ribosylation refers to the addition of one or more ADP-ribose units onto proteins post-translationally. This protein modification is often added by ADPribosyltransferases, commonly known as PARPs, but it can also be added by other enzymes, including sirtuins or bacterial toxins. While past literature has utilized a variety of methods to identify ADP-ribosylated proteins, recent proteomics studies bring the power of mass spectrometry to determine sites of the modification. To appreciate the diverse roles of ADPribosylation across the proteome, we have created ADPriboDB-a database of ADP-ribosylated proteins (http://ADPriboDB.leunglab.org). Each entry of ADPriboDB is annotated manually by at least two independent curators from the literature between January 1975 and July 2015. The current database includes over 12 400 protein entries from 459 publications, identifying 2389 unique proteins. Here, we describe the structure and the current state of ADPriboDB as well as the criteria for entry inclusion. Using this aggregate data, we identified a statistically significant enrichment of ADP-ribosylated proteins in non-membranous RNA granules. To our knowledge, ADPriboDB is the first publicly available database encapsulating ADP-ribosylated proteins identified from the past 40 years, with a hope to facilitate the research of both basic scientists and clinicians to better understand ADP-ribosylation at the molecular level.

Original languageEnglish (US)
Pages (from-to)D204-D209
JournalNucleic Acids Research
Volume45
Issue numberD1
DOIs
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Genetics

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