ADP-ribosyltransferase activity of mono- and multi-(ADP-ribosylated) choleragen.

J. Moss, S. J. Stanley, Paul A Watkins, M. Vaughan

Research output: Contribution to journalArticle

Abstract

Choleragen (cholera toxin) catalyzed the NAD-dependent auto-ADP-ribosyltation of its A1 peptide. The number of ADP-ribose moieties incorporated into ech A1 peptide was dependent on incubation conditions and time as well as toxin concentration. There was no evidence for the formation of poly(ADP-ribosylated) toxin. The formation of mono- and multi-(ADP-ribosylated) A1 peptides was prevented by the addition of arginine, an alternative ADP-ribose acceptor. Triton X-100 polyacrylamide gel electrophoresis separated the A1 peptide of choleragen from the B complex and multi-(ADP-ribosylated) A1 peptides from unmodified A1. The A1 peptides ADP-ribosylated in the presence of [32P]NAD contained approximately 1, 2, or 3 ADP-ribose molecules/23,500-dalton units; under the conditions used for electrophoresis, the mobilities of the A1 peptides were enhanced by incorporation of ADP-ribose. The mono- and multi-(ADP-ribosylated) A1 peptides catalyzed the NAD-dependent ADP-ribosylation of arginine methyl ester. The turnover numbers of the mono- and multi-(ADP-ribosylated) A1 peptides were consistently 30 to 50% higher than that of the A1 peptide from native toxin.

Original languageEnglish (US)
Pages (from-to)7835-7837
Number of pages3
JournalJournal of Biological Chemistry
Volume255
Issue number16
StatePublished - Aug 25 1980
Externally publishedYes

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ADP Ribose Transferases
Cholera Toxin
Adenosine Diphosphate
Peptides
Adenosine Diphosphate Ribose
NAD
Electrophoresis
Octoxynol
Polyacrylamide Gel Electrophoresis
Arginine

ASJC Scopus subject areas

  • Biochemistry

Cite this

ADP-ribosyltransferase activity of mono- and multi-(ADP-ribosylated) choleragen. / Moss, J.; Stanley, S. J.; Watkins, Paul A; Vaughan, M.

In: Journal of Biological Chemistry, Vol. 255, No. 16, 25.08.1980, p. 7835-7837.

Research output: Contribution to journalArticle

Moss, J. ; Stanley, S. J. ; Watkins, Paul A ; Vaughan, M. / ADP-ribosyltransferase activity of mono- and multi-(ADP-ribosylated) choleragen. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 16. pp. 7835-7837.
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