Adipose fatty acid oxidation is required for thermogenesis and potentiates oxidative stress-induced inflammation

Jieun Lee, Jessica M. Ellis, Michael J. Wolfgang

Research output: Contribution to journalArticlepeer-review

Abstract

To understand the contribution of adipose tissue fatty acid oxidation to whole-body metabolism, we generated mice with an adipose-specific knockout of carnitine palmitoyltransferase 2 (CPT2A-/-), an obligate step in mitochondrial long-chain fatty acid oxidation. CPT2A-/- mice became hypothermic after an acute cold challenge, and CPT2A-/- brown adipose tissue (BAT) failed to upregulate thermogenic genes in response to agonist-induced stimulation. The adipose-specific loss of CPT2 resulted in diet-dependent changes in adiposity but did not result in changes in body weight on low- or high-fat diets. Additionally, CPT2A-/- mice had suppressed high-fat diet-induced oxidative stress and inflammation in visceral white adipose tissue (WAT); however, high-fat diet-induced glucose intolerance was not improved. These data show that fatty acid oxidation is required for cold-induced thermogenesis in BAT and high-fat diet-induced oxidative stress and inflammation in WAT.

Original languageEnglish (US)
Pages (from-to)266-279
Number of pages14
JournalCell Reports
Volume10
Issue number2
DOIs
StatePublished - Jan 13 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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