Adhesion of tumour-infiltrating lymphocytes to endothelium: A phenotypic and functional analysis

D. H. Adams, J. R. Yannelli, W. Newman, T. Lawley, E. Ades, S. A. Rosenberg, S. Shaw

Research output: Contribution to journalArticle

Abstract

Efficacy of cancer immunotherapy with cultured tumour-infiltrating lymphocytes (TILs) depends upon infused TILs migrating into tumour-bearing tissue, in which they mediate an anti-tumour response. For TILs to enter a tumour, they must first bind to tumour endothelium, and this process depends on TILs expressing and regulating the function of relevant cell-surface receptors. We analysed the cell-surface phenotype and endothelial binding of TILs cultured from human melanoma and compared them with peripheral blood T cells and with allostimulated T cells cultured under similar conditions. Compared with peripheral blood T cells, TILs expressed high levels of five integrins, two other adhesion molecules, including the skin homing molecule CLA, and several activation markers and showed markedly enhanced integrin-mediated adhesion to a dermal microvascular endothelial cell line in vitro. Compared with the allostimulated T cells, TILs expressed higher levels of the cutaneous lymphocyte antigen (CLA), the adhesion molecule CD31 and the activation markers CD30 and CD69, but lower levels of several other adhesion and activation molecules. These phenotypic and functional properties of TILs should have complex effects on their migration in vivo. Expression of CLA, the skin homing receptor, may increase migration to melanoma (a skin cancer), whereas integrin activation may cause non-specific binding of TILs to other endothelium. Manipulation of the culture conditions in which TILs are expanded might result in a phenotype that is more conducive to selective tumour homing in vivo.

Original languageEnglish (US)
Pages (from-to)1421-1431
Number of pages11
JournalBritish Journal of Cancer
Volume75
Issue number10
StatePublished - 1997
Externally publishedYes

Fingerprint

Tumor-Infiltrating Lymphocytes
Endothelium
Skin
Integrins
T-Lymphocytes
Neoplasms
Lymphocytes
Antigens
Melanoma
Blood Cells
Phenotype
Cell Surface Receptors
Skin Neoplasms
Immunotherapy
Endothelial Cells

Keywords

  • Endothelium
  • Melanoma
  • Tumour-infiltrating lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Adams, D. H., Yannelli, J. R., Newman, W., Lawley, T., Ades, E., Rosenberg, S. A., & Shaw, S. (1997). Adhesion of tumour-infiltrating lymphocytes to endothelium: A phenotypic and functional analysis. British Journal of Cancer, 75(10), 1421-1431.

Adhesion of tumour-infiltrating lymphocytes to endothelium : A phenotypic and functional analysis. / Adams, D. H.; Yannelli, J. R.; Newman, W.; Lawley, T.; Ades, E.; Rosenberg, S. A.; Shaw, S.

In: British Journal of Cancer, Vol. 75, No. 10, 1997, p. 1421-1431.

Research output: Contribution to journalArticle

Adams, DH, Yannelli, JR, Newman, W, Lawley, T, Ades, E, Rosenberg, SA & Shaw, S 1997, 'Adhesion of tumour-infiltrating lymphocytes to endothelium: A phenotypic and functional analysis', British Journal of Cancer, vol. 75, no. 10, pp. 1421-1431.
Adams DH, Yannelli JR, Newman W, Lawley T, Ades E, Rosenberg SA et al. Adhesion of tumour-infiltrating lymphocytes to endothelium: A phenotypic and functional analysis. British Journal of Cancer. 1997;75(10):1421-1431.
Adams, D. H. ; Yannelli, J. R. ; Newman, W. ; Lawley, T. ; Ades, E. ; Rosenberg, S. A. ; Shaw, S. / Adhesion of tumour-infiltrating lymphocytes to endothelium : A phenotypic and functional analysis. In: British Journal of Cancer. 1997 ; Vol. 75, No. 10. pp. 1421-1431.
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