Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: Contributions of endothelial cell adhesion molecules

Bruce S. Bochner, Francis W. Luscinskas, Michael A. Gimbrone, Walter Newman, Sherry A. Sterbinsky, Claudia P. Derse-Anthony, David Klunk, Robert P. Schleimer

Research output: Contribution to journalArticle

Abstract

Cytokines such as interleukin 1 (IL-1) promote adhesiveness in human umbilical vein endothelial cells for leukocytes including basophils, eosinophils, and neutrophils, and induce expression of adherence molecules including ICAM-1 (intercellular adhesion molecule-1), ELAM-1 (endothelial-leukocyte adhesion molecule-1), and VCAM-1 (vascular cell adhesion molecule-1). In the present study, blocking monoclonal antibodies (mAb) recognizing ICAM-1, ELAM-1, and VCAM-1 have been used to compare their roles in IL-l-induced adhesion of human basophils, eosinophils, and neutrophils. IL-1 treatment of endothelial cell monolayers for 4 hours induced a four- to eight-fold increase in adhesion for each cell type. Treatment of endothelial cells with either anti-ICAM-1 or anti-ELAM-1 mAb inhibited IL-l-induced adherence of each cell type. In contrast, treatment with anti-VCAM-1 mAb inhibited basophil and eosinophil (but not neutrophil) adhesion, and was especially effective in blocking eosinophil adhesion. The effects of these mAb were at least additive. Indirect immunofluorescence and flow cytometry demonstrated expression of VLA-4α (very late activation antigen-4α, a counter-receptor for VCAM-1) on eosinophils and basophils but not on neutrophils. These data document distinct roles for ICAM-1, ELAM-1, and VCAM-1 during basophil, eosinophil, and neutrophil adhesion in vitro, and suggest a novel mechanism for the recruitment of eosinophils and basophils to sites of inflammation in vivo.

Original languageEnglish (US)
Pages (from-to)1553-1556
Number of pages4
JournalJournal of Experimental Medicine
Volume173
Issue number6
DOIs
StatePublished - Jun 1 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Bochner, B. S., Luscinskas, F. W., Gimbrone, M. A., Newman, W., Sterbinsky, S. A., Derse-Anthony, C. P., Klunk, D., & Schleimer, R. P. (1991). Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: Contributions of endothelial cell adhesion molecules. Journal of Experimental Medicine, 173(6), 1553-1556. https://doi.org/10.1084/jem.173.6.1553