This chapter focuses on the adhesion of eukaryotic cells to immobilized carbohydrates. To study the carbohydrate-directed control of cell behaviors, methods have been developed to immobilize various carbohydrates on otherwise inert synthetic surfaces and to analyze the responses of intact cells in contact with these surfaces. Various carbohydrates (including low molecular weight glycosides, glycopeptides, glycoproteins, polysaccharides, and glycolipids) are covalently attached or noncovalently (but stably) adsorbed to plastic surfaces. Hydrophilic carbohydrates are readily soluble in aqueous media and must be covalently attached to artificial surfaces for use as cell surface analogs. In contrast, amphipathic glycolipids are stably adsorbed to hydrophobic plastic surfaces for this purpose or are probed directly on thin-layer chromatography (TLC) plates. The chapter concludes with a discussion of cell adhesion to glycosphingolipids, separated on TLC plates.
ASJC Scopus subject areas
- Molecular Biology