Adhesion Molecules as Susceptibility Factors in Spontaneous Autoimmune Thyroiditis in the NOD-H2h4 Mouse

Raphael E. Bonita, Noel R. Rose, Linda Rasooly, Patrizio P Caturegli, C. Lynne Burek

Research output: Contribution to journalArticle

Abstract

Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of adhesion molecules only late in clinical disease. In this study, we examined the distribution and kinetics of expression of E-selectin, VCAM-1, LFA-1, and ICAM-1 in the NOD-H2h4 mouse, a model of spontaneous autoimmune thyroiditis accelerated by dietary iodine. Mice were fed 0.015% NaI in their drinking water for 2, 4, 6, 8, and 16 weeks, and thyroids were removed, serially sectioned, and stained in an avidin-biotin-peroxidase assay. We found a dramatic increase in E-selectin and VCAM-1 expression on intrathyroidal endothelial cells after 16 weeks of iodine treatment. In addition, we describe for the first time that thyrocytes from the NOD-H2h4 mouse, and the parental NOD, constitutively express ICAM-1 independent of iodine treatment and prior to mononuclear cell infiltration of the thyroid gland. ICAM-1 was not detected on the thyrocytes of other untreated strains of mice, implicating expression of this adhesion molecule as a critical event in the recruitment of inflammatory mononuclear cells to the thyroid.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalExperimental and Molecular Pathology
Volume73
Issue number3
DOIs
StatePublished - Dec 2002

Fingerprint

Autoimmune Thyroiditis
Inbred NOD Mouse
Intercellular Adhesion Molecule-1
Iodine
Thyroid Gland
E-Selectin
Adhesion
Vascular Cell Adhesion Molecule-1
Infiltration
Molecules
Lymphocyte Function-Associated Antigen-1
Avidin
Endothelial cells
Biotin
Drinking Water
Peroxidase
Assays
Animals
Hashimoto Disease
Kinetics

Keywords

  • Adhesion molecules
  • Autoimmune thyroiditis
  • ICAM-1
  • Immunohistochemistry
  • Iodine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Adhesion Molecules as Susceptibility Factors in Spontaneous Autoimmune Thyroiditis in the NOD-H2h4 Mouse. / Bonita, Raphael E.; Rose, Noel R.; Rasooly, Linda; Caturegli, Patrizio P; Burek, C. Lynne.

In: Experimental and Molecular Pathology, Vol. 73, No. 3, 12.2002, p. 155-163.

Research output: Contribution to journalArticle

Bonita, Raphael E. ; Rose, Noel R. ; Rasooly, Linda ; Caturegli, Patrizio P ; Burek, C. Lynne. / Adhesion Molecules as Susceptibility Factors in Spontaneous Autoimmune Thyroiditis in the NOD-H2h4 Mouse. In: Experimental and Molecular Pathology. 2002 ; Vol. 73, No. 3. pp. 155-163.
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