Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Terrence M. Dobrowsky; S. Alireza Rabi; Rebecca Nedellec; Brian R. Daniels; James I. Mullins; Donald E. Mosier; Robert F. Siliciano; Denis Wirtz

doi:10.1038/srep03014

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Terrence M. Dobrowsky, S. Alireza Rabi, Rebecca Nedellec, Brian R. Daniels, James I. Mullins, Donald E. Mosier, Robert F. Siliciano, Denis Wirtz

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In about half of patients infected with HIV-1 subtype B, viral populations shift from utilizing the transmembrane protein CCR5 to CXCR4, as well as or instead of CCR5, during late stage progression of the disease. How the relative adhesion efficiency and fusion competency of the viral Env proteins relate to infection during this transition is not well understood. Using a virus-cell fusion assay and live-cell single-molecule force spectroscopy, we compare the entry competency of viral clones to tensile strengths of the individual Env-receptor bonds of Env proteins obtained from a HIV-1 infected patient prior to and during coreceptor switching. The results suggest that the genetic determinants of viral entry were predominantly enriched in the C3, HR1 and CD regions rather than V3. Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors.

Original language	English (US)
Article number	3014
Journal	Scientific reports
Volume	3
DOIs	https://doi.org/10.1038/srep03014
State	Published - 2013

ASJC Scopus subject areas

General

Access to Document

10.1038/srep03014

Cite this

@article{f60ac1caab914fa587a3c8ef0ddffea6,

title = "Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins",

abstract = "In about half of patients infected with HIV-1 subtype B, viral populations shift from utilizing the transmembrane protein CCR5 to CXCR4, as well as or instead of CCR5, during late stage progression of the disease. How the relative adhesion efficiency and fusion competency of the viral Env proteins relate to infection during this transition is not well understood. Using a virus-cell fusion assay and live-cell single-molecule force spectroscopy, we compare the entry competency of viral clones to tensile strengths of the individual Env-receptor bonds of Env proteins obtained from a HIV-1 infected patient prior to and during coreceptor switching. The results suggest that the genetic determinants of viral entry were predominantly enriched in the C3, HR1 and CD regions rather than V3. Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors.",

author = "Dobrowsky, {Terrence M.} and Rabi, {S. Alireza} and Rebecca Nedellec and Daniels, {Brian R.} and Mullins, {James I.} and Mosier, {Donald E.} and Siliciano, {Robert F.} and Denis Wirtz",

note = "Funding Information: This work was supported by NIH grants GM084204, CA143868, and CA85839 (DW), AI52778 (DEM) and AI47734 (JIM), the University of Washington Center for AIDS Research Computational Biology Core (AI 27757), and the Howard Hughes Medical Institute. We thank Dr. Shyam B. Khatau for fruitful discussions and editorial advice.",

year = "2013",

doi = "10.1038/srep03014",

language = "English (US)",

volume = "3",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

AU - Dobrowsky, Terrence M.

AU - Rabi, S. Alireza

AU - Nedellec, Rebecca

AU - Daniels, Brian R.

AU - Mullins, James I.

AU - Mosier, Donald E.

AU - Siliciano, Robert F.

AU - Wirtz, Denis

N1 - Funding Information: This work was supported by NIH grants GM084204, CA143868, and CA85839 (DW), AI52778 (DEM) and AI47734 (JIM), the University of Washington Center for AIDS Research Computational Biology Core (AI 27757), and the Howard Hughes Medical Institute. We thank Dr. Shyam B. Khatau for fruitful discussions and editorial advice.

PY - 2013

Y1 - 2013

N2 - In about half of patients infected with HIV-1 subtype B, viral populations shift from utilizing the transmembrane protein CCR5 to CXCR4, as well as or instead of CCR5, during late stage progression of the disease. How the relative adhesion efficiency and fusion competency of the viral Env proteins relate to infection during this transition is not well understood. Using a virus-cell fusion assay and live-cell single-molecule force spectroscopy, we compare the entry competency of viral clones to tensile strengths of the individual Env-receptor bonds of Env proteins obtained from a HIV-1 infected patient prior to and during coreceptor switching. The results suggest that the genetic determinants of viral entry were predominantly enriched in the C3, HR1 and CD regions rather than V3. Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors.

AB - In about half of patients infected with HIV-1 subtype B, viral populations shift from utilizing the transmembrane protein CCR5 to CXCR4, as well as or instead of CCR5, during late stage progression of the disease. How the relative adhesion efficiency and fusion competency of the viral Env proteins relate to infection during this transition is not well understood. Using a virus-cell fusion assay and live-cell single-molecule force spectroscopy, we compare the entry competency of viral clones to tensile strengths of the individual Env-receptor bonds of Env proteins obtained from a HIV-1 infected patient prior to and during coreceptor switching. The results suggest that the genetic determinants of viral entry were predominantly enriched in the C3, HR1 and CD regions rather than V3. Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors.

UR - http://www.scopus.com/inward/record.url?scp=84886499794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886499794&partnerID=8YFLogxK

U2 - 10.1038/srep03014

DO - 10.1038/srep03014

M3 - Article

C2 - 24145278

AN - SCOPUS:84886499794

SN - 2045-2322

VL - 3

JO - Scientific reports

JF - Scientific reports

M1 - 3014

ER -

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this