Adherence of Helicobacter pylori to cultured human gastric epithelial cells

D. T. Smoot; J. H. Resau; T. Naab; B. C. Desbordes; T. Gilliam; K. Bull-Henry; S. B. Curry; J. Nidiry; J. Sewchand; K. Mills-Robertson; K. Frontin; E. Abebe; M. Dillon; G. R. Chippendale; P. C. Phelps; V. F. Scott; H. L.T. Mobley

Adherence of Helicobacter pylori to cultured human gastric epithelial cells

D. T. Smoot, J. H. Resau, T. Naab, B. C. Desbordes, T. Gilliam, K. Bull-Henry, S. B. Curry, J. Nidiry, J. Sewchand, K. Mills-Robertson, K. Frontin, E. Abebe, M. Dillon, G. R. Chippendale, P. C. Phelps, V. F. Scott, H. L.T. Mobley

Research output: Contribution to journal › Comment/debate › peer-review

79 Scopus citations

Abstract

Experiments were performed to demonstrate that adherence of Helicobacter pylori to gastric epithelial cells causes alterations in the cell cytoskeleton. H. pylori intimately attached to cultured human gastric epithelial cells on small cellular projections, while there was no intimate association of H. pylori with cultured human esophageal epithelial cells. Fluorescein-conjugated phalloidin staining of gastric epithelial cells showed that H. pylori adherence stimulated actin polymerization; this stimulation was not observed with esophageal cells. Also, this organism's selectivity for gastric mucosa was supported by rare binding of bacteria to esophageal epithelial cells and gastric fibroblasts.

Original language	English (US)
Pages (from-to)	350-355
Number of pages	6
Journal	Infection and immunity
Volume	61
Issue number	1
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

Cite this

Smoot, D. T., Resau, J. H., Naab, T., Desbordes, B. C., Gilliam, T., Bull-Henry, K., Curry, S. B., Nidiry, J., Sewchand, J., Mills-Robertson, K., Frontin, K., Abebe, E., Dillon, M., Chippendale, G. R., Phelps, P. C., Scott, V. F., & Mobley, H. L. T. (1993). Adherence of Helicobacter pylori to cultured human gastric epithelial cells. Infection and immunity, 61(1), 350-355.

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title = "Adherence of Helicobacter pylori to cultured human gastric epithelial cells",

abstract = "Experiments were performed to demonstrate that adherence of Helicobacter pylori to gastric epithelial cells causes alterations in the cell cytoskeleton. H. pylori intimately attached to cultured human gastric epithelial cells on small cellular projections, while there was no intimate association of H. pylori with cultured human esophageal epithelial cells. Fluorescein-conjugated phalloidin staining of gastric epithelial cells showed that H. pylori adherence stimulated actin polymerization; this stimulation was not observed with esophageal cells. Also, this organism's selectivity for gastric mucosa was supported by rare binding of bacteria to esophageal epithelial cells and gastric fibroblasts.",

author = "Smoot, {D. T.} and Resau, {J. H.} and T. Naab and Desbordes, {B. C.} and T. Gilliam and K. Bull-Henry and Curry, {S. B.} and J. Nidiry and J. Sewchand and K. Mills-Robertson and K. Frontin and E. Abebe and M. Dillon and Chippendale, {G. R.} and Phelps, {P. C.} and Scott, {V. F.} and Mobley, {H. L.T.}",

year = "1993",

language = "English (US)",

volume = "61",

pages = "350--355",

journal = "Infection and immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "1",

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TY - JOUR

T1 - Adherence of Helicobacter pylori to cultured human gastric epithelial cells

AU - Smoot, D. T.

AU - Resau, J. H.

AU - Naab, T.

AU - Desbordes, B. C.

AU - Gilliam, T.

AU - Bull-Henry, K.

AU - Curry, S. B.

AU - Nidiry, J.

AU - Sewchand, J.

AU - Mills-Robertson, K.

AU - Frontin, K.

AU - Abebe, E.

AU - Dillon, M.

AU - Chippendale, G. R.

AU - Phelps, P. C.

AU - Scott, V. F.

AU - Mobley, H. L.T.

PY - 1993

Y1 - 1993

N2 - Experiments were performed to demonstrate that adherence of Helicobacter pylori to gastric epithelial cells causes alterations in the cell cytoskeleton. H. pylori intimately attached to cultured human gastric epithelial cells on small cellular projections, while there was no intimate association of H. pylori with cultured human esophageal epithelial cells. Fluorescein-conjugated phalloidin staining of gastric epithelial cells showed that H. pylori adherence stimulated actin polymerization; this stimulation was not observed with esophageal cells. Also, this organism's selectivity for gastric mucosa was supported by rare binding of bacteria to esophageal epithelial cells and gastric fibroblasts.

AB - Experiments were performed to demonstrate that adherence of Helicobacter pylori to gastric epithelial cells causes alterations in the cell cytoskeleton. H. pylori intimately attached to cultured human gastric epithelial cells on small cellular projections, while there was no intimate association of H. pylori with cultured human esophageal epithelial cells. Fluorescein-conjugated phalloidin staining of gastric epithelial cells showed that H. pylori adherence stimulated actin polymerization; this stimulation was not observed with esophageal cells. Also, this organism's selectivity for gastric mucosa was supported by rare binding of bacteria to esophageal epithelial cells and gastric fibroblasts.

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UR - http://www.scopus.com/inward/citedby.url?scp=0027459798&partnerID=8YFLogxK

M3 - Comment/debate

C2 - 8418061

AN - SCOPUS:0027459798

SN - 0019-9567

VL - 61

SP - 350

EP - 355

JO - Infection and immunity

JF - Infection and immunity

IS - 1

ER -

Adherence of Helicobacter pylori to cultured human gastric epithelial cells

Abstract

ASJC Scopus subject areas

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