Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay

C. A. Lyman, E. Navarro, K. F. Garrett, D. D. Roberts, P. A. Pizzo, T. J. Walsh

Research output: Contribution to journalArticle

Abstract

In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.

Original languageEnglish (US)
Pages (from-to)255-259
Number of pages5
JournalMycoses
Volume42
Issue number4
DOIs
StatePublished - Jul 27 1999

Keywords

  • Adherence
  • Candida
  • Tissue explant assay
  • Uroepithelium

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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    Lyman, C. A., Navarro, E., Garrett, K. F., Roberts, D. D., Pizzo, P. A., & Walsh, T. J. (1999). Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay. Mycoses, 42(4), 255-259. https://doi.org/10.1046/j.1439-0507.1999.00453.x