Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay

C. A. Lyman; E. Navarro; K. F. Garrett; D. D. Roberts; P. A. Pizzo; T. J. Walsh

doi:10.1046/j.1439-0507.1999.00453.x

Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay

C. A. Lyman, E. Navarro, K. F. Garrett, D. D. Roberts, P. A. Pizzo, T. J. Walsh

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.

Original language	English (US)
Pages (from-to)	255-259
Number of pages	5
Journal	Mycoses
Volume	42
Issue number	4
DOIs	https://doi.org/10.1046/j.1439-0507.1999.00453.x
State	Published - 1999
Externally published	Yes

Keywords

Adherence
Candida
Tissue explant assay
Uroepithelium

ASJC Scopus subject areas

Dermatology
Infectious Diseases

Access to Document

10.1046/j.1439-0507.1999.00453.x

Cite this

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title = "Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay",

abstract = "In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.",

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T2 - Development and application of a tissue explant assay

AU - Lyman, C. A.

AU - Navarro, E.

AU - Garrett, K. F.

AU - Roberts, D. D.

AU - Pizzo, P. A.

AU - Walsh, T. J.

PY - 1999

Y1 - 1999

N2 - In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.

AB - In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.

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KW - Tissue explant assay

KW - Uroepithelium

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Adherence of Candida albicans to bladder mucosa: Development and application of a tissue explant assay

Abstract

Keywords

ASJC Scopus subject areas

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