Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores

Saskia Vanes, Kiran J. Virdy, Geoffrey S. Pitt, Marcel Meimaf, Todd W. Sands, Peter N Devreotes, David A. Cotter, Pauline Schaap

Research output: Contribution to journalArticle

Abstract

Dictyostelium cells express a G-protein-coupled adenylyl cyclase, ACA, during aggregation and an atypical adenylyl cyclase, ACG, in mature spores. The ACG gene was disrupted by homologous recombination. acg- cells developed into normal fruiting bodies with viable spores, but spore germination was no longer inhibited by high osmolarity, a fairly universal constraint for spore and seed germination. ACG activity, measured in aca-/ACG cells, was strongly stimulated by high osmolarity with optimal stimulation occurring at 200 milliosmolar. RdeC mutants, which display unrestrained protein kinase A (PKA) activity and a cell line, which overexpresses PKA under a prespore specific promoter, germinate very poorly, both at high and low osmolarity. These data indicate that ACG is an osmosensor controlling spore germination through activation of protein kinase A.

Original languageEnglish (US)
Pages (from-to)23623-23625
Number of pages3
JournalJournal of Biological Chemistry
Volume271
Issue number39
DOIs
StatePublished - 1996

Fingerprint

Dictyostelium
Germination
Cyclic AMP-Dependent Protein Kinases
Spores
Adenylyl Cyclases
Osmolar Concentration
GTP-Binding Proteins
Seed
Agglomeration
Genes
Chemical activation
Cells
Homologous Recombination
Seeds
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores. / Vanes, Saskia; Virdy, Kiran J.; Pitt, Geoffrey S.; Meimaf, Marcel; Sands, Todd W.; Devreotes, Peter N; Cotter, David A.; Schaap, Pauline.

In: Journal of Biological Chemistry, Vol. 271, No. 39, 1996, p. 23623-23625.

Research output: Contribution to journalArticle

Vanes, S, Virdy, KJ, Pitt, GS, Meimaf, M, Sands, TW, Devreotes, PN, Cotter, DA & Schaap, P 1996, 'Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores', Journal of Biological Chemistry, vol. 271, no. 39, pp. 23623-23625. https://doi.org/10.1074/jbc.271.39.23623
Vanes, Saskia ; Virdy, Kiran J. ; Pitt, Geoffrey S. ; Meimaf, Marcel ; Sands, Todd W. ; Devreotes, Peter N ; Cotter, David A. ; Schaap, Pauline. / Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 39. pp. 23623-23625.
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AU - Vanes, Saskia

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AU - Pitt, Geoffrey S.

AU - Meimaf, Marcel

AU - Sands, Todd W.

AU - Devreotes, Peter N

AU - Cotter, David A.

AU - Schaap, Pauline

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AB - Dictyostelium cells express a G-protein-coupled adenylyl cyclase, ACA, during aggregation and an atypical adenylyl cyclase, ACG, in mature spores. The ACG gene was disrupted by homologous recombination. acg- cells developed into normal fruiting bodies with viable spores, but spore germination was no longer inhibited by high osmolarity, a fairly universal constraint for spore and seed germination. ACG activity, measured in aca-/ACG cells, was strongly stimulated by high osmolarity with optimal stimulation occurring at 200 milliosmolar. RdeC mutants, which display unrestrained protein kinase A (PKA) activity and a cell line, which overexpresses PKA under a prespore specific promoter, germinate very poorly, both at high and low osmolarity. These data indicate that ACG is an osmosensor controlling spore germination through activation of protein kinase A.

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