Adenylyl cyclase A expression is tip-specific in Dictyostelium slugs and directs StatA nuclear translocation and CudA gene expression

Irene Verkerke Van Wijk, Masashi Fukuzawa, Peter N. Devreotes, Pauline Schaap

Research output: Contribution to journalArticle

Abstract

cAMP oscillations, generated by adenylyl cyclase A (ACA), coordinate cell aggregation in Dictyostelium and have also been implicated in organizer function during multicellular development. We used a gene fusion of the ACA promoter with a labile lacZ derivative to study the expression pattern of ACA. During aggregation, most cells expressed ACA, but thereafter expression was lost in all cells except those of the anterior tip. Before aggregation, ACA transcription was strongly upregulated by nanomolar cAMP pulses. Postaggregative transcription was sustained by nanomolar cAMP pulses, but downregulated by a continuous micromolar cAMP stimulus and by the stalk-cell-inducing factor DIF. Earlier work showed that the transcription factor StatA displays tip-specific nuclear translocation and directs tip-specific expression of the nuclear protein CudA, which is essential for culmination. Both StatA and CudA were present in nuclei throughout the entire slug in an aca null mutant that expresses ACA from the constitutive actin15 promoter. This suggests that the tip-specific expression of ACA directs tip-specific nuclear translocation of StatA and tip-specific expression of CudA.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalDevelopmental biology
Volume234
Issue number1
DOIs
StatePublished - Jun 1 2001

Keywords

  • Adenylyl cyclase A
  • Dictyostelium discoideum
  • Organizer
  • STAT transcription factor
  • cAMP oscillations

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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