TY - JOUR
T1 - Adenylyl cyclase A expression is tip-specific in Dictyostelium slugs and directs StatA nuclear translocation and CudA gene expression
AU - Wijk, Irene Verkerke Van
AU - Fukuzawa, Masashi
AU - Devreotes, Peter N.
AU - Schaap, Pauline
N1 - Funding Information:
We thank Harry MacWilliams for his kind gift of the psA-ubi-ala-gal and psA-ubi-ile-gal constructs and Jeff Williams for stimulating discussions. We are grateful to Iris Adrian for transformation of the statA− and cudA− mutants with the ACA-ile-gal vector and to Tomoaki Abe for expert advice and assistance with confocal laser microscopy. This research was supported by Grant 805-31-051 from the Life Sciences Foundation of The Netherlands Organisation for Scientific Research and by Wellcome Trust University Award Grant 057137.
PY - 2001/6/1
Y1 - 2001/6/1
N2 - cAMP oscillations, generated by adenylyl cyclase A (ACA), coordinate cell aggregation in Dictyostelium and have also been implicated in organizer function during multicellular development. We used a gene fusion of the ACA promoter with a labile lacZ derivative to study the expression pattern of ACA. During aggregation, most cells expressed ACA, but thereafter expression was lost in all cells except those of the anterior tip. Before aggregation, ACA transcription was strongly upregulated by nanomolar cAMP pulses. Postaggregative transcription was sustained by nanomolar cAMP pulses, but downregulated by a continuous micromolar cAMP stimulus and by the stalk-cell-inducing factor DIF. Earlier work showed that the transcription factor StatA displays tip-specific nuclear translocation and directs tip-specific expression of the nuclear protein CudA, which is essential for culmination. Both StatA and CudA were present in nuclei throughout the entire slug in an aca null mutant that expresses ACA from the constitutive actin15 promoter. This suggests that the tip-specific expression of ACA directs tip-specific nuclear translocation of StatA and tip-specific expression of CudA.
AB - cAMP oscillations, generated by adenylyl cyclase A (ACA), coordinate cell aggregation in Dictyostelium and have also been implicated in organizer function during multicellular development. We used a gene fusion of the ACA promoter with a labile lacZ derivative to study the expression pattern of ACA. During aggregation, most cells expressed ACA, but thereafter expression was lost in all cells except those of the anterior tip. Before aggregation, ACA transcription was strongly upregulated by nanomolar cAMP pulses. Postaggregative transcription was sustained by nanomolar cAMP pulses, but downregulated by a continuous micromolar cAMP stimulus and by the stalk-cell-inducing factor DIF. Earlier work showed that the transcription factor StatA displays tip-specific nuclear translocation and directs tip-specific expression of the nuclear protein CudA, which is essential for culmination. Both StatA and CudA were present in nuclei throughout the entire slug in an aca null mutant that expresses ACA from the constitutive actin15 promoter. This suggests that the tip-specific expression of ACA directs tip-specific nuclear translocation of StatA and tip-specific expression of CudA.
KW - Adenylyl cyclase A
KW - Dictyostelium discoideum
KW - Organizer
KW - STAT transcription factor
KW - cAMP oscillations
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U2 - 10.1006/dbio.2001.0232
DO - 10.1006/dbio.2001.0232
M3 - Article
C2 - 11356026
AN - SCOPUS:0035371364
SN - 0012-1606
VL - 234
SP - 151
EP - 160
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -