Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels

Antonio J. Carrasco, Petras P. Dzeja, Alexey E. Alekseev, Darko Pucar, Leonid V. Zingman, M. Roselle Abraham, Denice Hodgson, Martin Bienengraeber, Michel Puceat, Edwin Janssen, Be Wieringa, Andre Terzic

Research output: Contribution to journalArticle

Abstract

Transduction of energetic signals into membrane electrical events governs vital cellular functions, ranging from hormone secretion and cytoprotection to appetite control and hair growth. Central to the regulation of such diverse cellular processes are the metabolism sensing ATP-sensitive K+ (KATP) channels. However, the mechanism that communicates metabolic signals and integrates cellular energetics with KATP channel-dependent membrane excitability remains elusive. Here, we identify that the response of KATP channels to metabolic challenge is regulated by adenylate kinase phosphotransfer. Adenylate kinase associates with the KATP channel complex, anchoring cellular phosphotransfer networks and facilitating delivery of mitochondrial signals to the membrane environment. Deletion of the adenylate kinase gene compromised nucleotide exchange at the channel site and impeded communication between mitochondria and KATP channels, rendering cellular metabolic sensing defective. Assigning a signal processing role to adenylate kinase identifies a phosphorelay mechanism essential for efficient coupling of cellular energetics with KATP channels and associated functions.

Original languageEnglish (US)
Pages (from-to)7623-7628
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number13
DOIs
StatePublished - Jun 19 2001
Externally publishedYes

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Adenylate Kinase
KATP Channels
Membranes
Cytoprotection
Appetite
Signal Transduction
Mitochondria
Nucleotides
Adenosine Triphosphate
Communication
Hormones
Growth
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels. / Carrasco, Antonio J.; Dzeja, Petras P.; Alekseev, Alexey E.; Pucar, Darko; Zingman, Leonid V.; Abraham, M. Roselle; Hodgson, Denice; Bienengraeber, Martin; Puceat, Michel; Janssen, Edwin; Wieringa, Be; Terzic, Andre.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 13, 19.06.2001, p. 7623-7628.

Research output: Contribution to journalArticle

Carrasco, AJ, Dzeja, PP, Alekseev, AE, Pucar, D, Zingman, LV, Abraham, MR, Hodgson, D, Bienengraeber, M, Puceat, M, Janssen, E, Wieringa, B & Terzic, A 2001, 'Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 13, pp. 7623-7628. https://doi.org/10.1073/pnas.121038198
Carrasco, Antonio J. ; Dzeja, Petras P. ; Alekseev, Alexey E. ; Pucar, Darko ; Zingman, Leonid V. ; Abraham, M. Roselle ; Hodgson, Denice ; Bienengraeber, Martin ; Puceat, Michel ; Janssen, Edwin ; Wieringa, Be ; Terzic, Andre. / Adenylate kinase phosphotransfer communicates cellular energetic signals to ATP-sensitive potassium channels. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 13. pp. 7623-7628.
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