Abstract
Adenovirus particles canbeengineeredtodisplay exogenous peptides on their surfaces by modification of viral capsid proteins, and particles that display pathogen-derived peptides can induce protective immunity. We constructed viable recombinant adenoviruses that display B-cell epitopes from the Plasmodium falciparum circum sporozoite protein (PfCSP) in the major adenovirus capsid protein, hexon. Recombinants induced high-titer antibodies against CSP when injected intraperitoneally into mice. Serum obtained from immunized mice recognized both recombinant PfCSP protein and P. falciparum sporozoites, and neutralized P. falciparum sporozoites in vitro. Replicating adenovirus vaccines have provided economical protection against adenovirus disease for over three decades. The recombinants described here may provide a path to an affordable malaria vaccine in the developing world.
Original language | English (US) |
---|---|
Pages (from-to) | 1683-1689 |
Number of pages | 7 |
Journal | Vaccine |
Volume | 29 |
Issue number | 8 |
DOIs | |
State | Published - Feb 11 2011 |
Keywords
- Capsid display
- Immunogenicity
- Malaria vaccine
- Recombinant adenovirus
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases