Adenovirus-mediated expression of aquaporin-5 in epithelial cells

Christine Delporte, Brian C. O'Connell, Xinjun He, Indu S. Ambudkar, Peter Agre, Bruce J. Baum

Research output: Contribution to journalArticle

Abstract

A recombinant adenovirus coding for rat aquaporin-5 was constructed and plaque purified. The recombinant adenovirus (AdrAQP5) mediated the expression of aquaporin-5 in rat and human salivary cell lines and in dog kidney cells in vitro as demonstrated by Northern blot and Western blot analyses, and by confocal microscopy after immunofluorescent labeling. In kidney cells, expression of the transgene was optimal if cells were infected at their basolateral surface, a phenomenon associated with the distribution of integrin receptors on these cells. The expressed aquaporin-5 protein was functionally active because viral-mediated gene transfer resulted in a significant increase in the osmotically directed net fluid secretion rate across monolayers of kidney cells. AdrAQP5 should provide an efficient and useful means to impart facilitated water permeability to cells lacking such a pathway.

Original languageEnglish (US)
Pages (from-to)22070-22075
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number36
DOIs
StatePublished - Sep 25 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Delporte, C., O'Connell, B. C., He, X., Ambudkar, I. S., Agre, P., & Baum, B. J. (1996). Adenovirus-mediated expression of aquaporin-5 in epithelial cells. Journal of Biological Chemistry, 271(36), 22070-22075. https://doi.org/10.1074/jbc.271.36.22070