Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth

Pamarthi M. Mohan, Shravan K. Chintala, Sanjeeva Mohanam, Candece L. Gladson, Eui So Kim, Ziya L. Gokaslan, Sajani S. Lakka, Jack A. Roth, Bingliang Fang, Raymond Sawaya, Athanassios P. Kyritsis, Jasti S. Rao

Research output: Contribution to journalArticle

Abstract

The urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) play important roles in the proteolytic cascade involved in the invasiveness of gliomas and other invasive tumors. High-level expression of uPAR has been correlated with high-grade glioma cell lines and tumors. We report here that down-regulating uPAR levels by antisense strategy using an adenovirus construct (Ad-uPAR) inhibited glioma invasion in Matrigel and spheroid in vitro models. s.c. (U87-MG) and intracranial (SNB19) injections of Ad-uPAR- infected glioma cells did not produce tumors in nude mice. However, injection of the Ad-uPAR construct into previously established s.c. U87-MG tumors in nude mice caused regression of those tumors. Our results support the therapeutic potential of targeting the uPA-uPAR system for the treatment of gliomas and other cancers.

Original languageEnglish (US)
Pages (from-to)3369-3373
Number of pages5
JournalCancer Research
Volume59
Issue number14
StatePublished - Jul 15 1999
Externally publishedYes

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Urokinase Plasminogen Activator Receptors
Adenoviridae
Glioma
Growth
Genes
Neoplasms
Nude Mice
Injections
Urokinase-Type Plasminogen Activator
Tumor Cell Line
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mohan, P. M., Chintala, S. K., Mohanam, S., Gladson, C. L., Kim, E. S., Gokaslan, Z. L., ... Rao, J. S. (1999). Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth. Cancer Research, 59(14), 3369-3373.

Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth. / Mohan, Pamarthi M.; Chintala, Shravan K.; Mohanam, Sanjeeva; Gladson, Candece L.; Kim, Eui So; Gokaslan, Ziya L.; Lakka, Sajani S.; Roth, Jack A.; Fang, Bingliang; Sawaya, Raymond; Kyritsis, Athanassios P.; Rao, Jasti S.

In: Cancer Research, Vol. 59, No. 14, 15.07.1999, p. 3369-3373.

Research output: Contribution to journalArticle

Mohan, PM, Chintala, SK, Mohanam, S, Gladson, CL, Kim, ES, Gokaslan, ZL, Lakka, SS, Roth, JA, Fang, B, Sawaya, R, Kyritsis, AP & Rao, JS 1999, 'Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth', Cancer Research, vol. 59, no. 14, pp. 3369-3373.
Mohan PM, Chintala SK, Mohanam S, Gladson CL, Kim ES, Gokaslan ZL et al. Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth. Cancer Research. 1999 Jul 15;59(14):3369-3373.
Mohan, Pamarthi M. ; Chintala, Shravan K. ; Mohanam, Sanjeeva ; Gladson, Candece L. ; Kim, Eui So ; Gokaslan, Ziya L. ; Lakka, Sajani S. ; Roth, Jack A. ; Fang, Bingliang ; Sawaya, Raymond ; Kyritsis, Athanassios P. ; Rao, Jasti S. / Adenovirus-mediated delivery of antisense gene to urokinase-type plasminogen activator receptor suppresses glioma invasion and tumor growth. In: Cancer Research. 1999 ; Vol. 59, No. 14. pp. 3369-3373.
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