Adenovirus DNA replication in vitro: Origin and direction of daughter strand synthesis

Mark D. Challberg, Thomas J. Kelly

Research output: Contribution to journalArticle

Abstract

We have characterized a soluble enzyme system from adenovirus-infected cells that is capable of replicating exogenously added adenovirus DNA in vitro. Maximal DNA synthesis is observed when DNA-protein complex, isolated from purified adenovirus virions, is added as template. Under these conditions DNA replication starts at or near either end of the template. Daughter strand synthesis then proceeds in the 5′ to 3′ direction displacing the parental strand of the same polarity. Thus, the r daughter strand is synthesized from right to left on the conventional map of the adenovirus genome, and the l daughter strand is synthesized from left to right. This course of events is the same as that which occurs during adenovirus DNA replication in vivo. In contrast, when deproteinized adenovirus DNA is added to the in vitro system, the limited DNA synthesis that is observed appears to be due to a repair-like reaction. In particular, synthesis can begin at many sites within the template, and the synthetic product consists largely of short DNA chains that are covalently linked to template DNA strands.

Original languageEnglish (US)
Pages (from-to)999-1012
Number of pages14
JournalJournal of molecular biology
Volume135
Issue number4
DOIs
StatePublished - Dec 25 1979

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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