TY - JOUR
T1 - Adenovirus DNA replication in vitro
T2 - Origin and direction of daughter strand synthesis
AU - Challberg, Mark D.
AU - Kelly, Thomas J.
N1 - Funding Information:
WC thank Michael Stern for expert technical assistance and Stephen Desiderio and Gary Ketner for helpful discussions. This work was supported by a United States Public Health Services research grant from the National Cancer Institute.
PY - 1979/12/25
Y1 - 1979/12/25
N2 - We have characterized a soluble enzyme system from adenovirus-infected cells that is capable of replicating exogenously added adenovirus DNA in vitro. Maximal DNA synthesis is observed when DNA-protein complex, isolated from purified adenovirus virions, is added as template. Under these conditions DNA replication starts at or near either end of the template. Daughter strand synthesis then proceeds in the 5′ to 3′ direction displacing the parental strand of the same polarity. Thus, the r daughter strand is synthesized from right to left on the conventional map of the adenovirus genome, and the l daughter strand is synthesized from left to right. This course of events is the same as that which occurs during adenovirus DNA replication in vivo. In contrast, when deproteinized adenovirus DNA is added to the in vitro system, the limited DNA synthesis that is observed appears to be due to a repair-like reaction. In particular, synthesis can begin at many sites within the template, and the synthetic product consists largely of short DNA chains that are covalently linked to template DNA strands.
AB - We have characterized a soluble enzyme system from adenovirus-infected cells that is capable of replicating exogenously added adenovirus DNA in vitro. Maximal DNA synthesis is observed when DNA-protein complex, isolated from purified adenovirus virions, is added as template. Under these conditions DNA replication starts at or near either end of the template. Daughter strand synthesis then proceeds in the 5′ to 3′ direction displacing the parental strand of the same polarity. Thus, the r daughter strand is synthesized from right to left on the conventional map of the adenovirus genome, and the l daughter strand is synthesized from left to right. This course of events is the same as that which occurs during adenovirus DNA replication in vivo. In contrast, when deproteinized adenovirus DNA is added to the in vitro system, the limited DNA synthesis that is observed appears to be due to a repair-like reaction. In particular, synthesis can begin at many sites within the template, and the synthetic product consists largely of short DNA chains that are covalently linked to template DNA strands.
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U2 - 10.1016/0022-2836(79)90524-2
DO - 10.1016/0022-2836(79)90524-2
M3 - Article
C2 - 231685
AN - SCOPUS:0018634639
SN - 0022-2836
VL - 135
SP - 999
EP - 1012
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 4
ER -