Adenovirus DNA replication in vitro: Characterization of a protein covalently linked to nascent DNA strands

M. D. Challberg, S. V. Desiderio, T. J. Kelly

Research output: Contribution to journalArticlepeer-review

Abstract

The 5' terminus of each nascent daughter strand of adenovirus DNA replicated in vitro is covalently linked to a protein with an apparent M(r) of 80,000. This protein may represent a precursor to the 55,000-dalton protein known to be linked to the 5' ends of mature adenovirus DNA strands. Partial proteolysis experiments indicate that the 80,000-dalton and 55,000-dalton proteins are structurally related. Furthermore, both proteins are attached to DNA by the same linkage: a phosphodiester bond between the β-OH of a serine residue in the protein and the 5'-OH of the terminal deoxycytidine residue of the DNA. The role of the 80,000-dalton protein in adenovirus DNA replication is not yet clear, although one reasonable possibility is that it serves as the primer for daughter strand synthesis.

Original languageEnglish (US)
Pages (from-to)5105-5109
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number9 II
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • General

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