Adenovirally encoded prohormone convertase-1 functions in atrial myocyte large dense core vesicles

Ruth Marx, Richard E. Mains

Research output: Contribution to journalArticle

Abstract

Bioactive peptides are usually synthesized as inactive precursor proteins that yield bioactive products only afar specific biosynthetic processing events. Large dense core vesicles (LDCV) are usually the site of storage of mature peptides. Atrial myocyte LDCV are rather unique in their storage of intact prohormone, proatrial natriuretic factor (pro-ANF), with no storage of cleaved products. To investigate whether the lack of intracellular cleavage of pro-ANF is due to the absence of prohormone convertases (PCs) from the atrial granules or to other factors, we expressed PC1 in atrial myocyte cultures using a recombinant adenovirus vector. Pro-PC1 protein was processed to mature PC1 and to the COOH-terminally shortened neuroendocrine- specific form of PC1 and rapidly secreted. Integral membrane forms of peptidylglycine α-amidating monooxygenase (PAM) were processed by PC1, and two primary products were secreted: a monofunctional monooxygenase and a larger bifunctional form. The cleaved PAM products were stored in LDCV, as secretion of PAM-derived products was stimulatable. In addition, pro-ANF was processed to ANF within PC 1-expressing cells. In primary atrial myocytes, vitally encoded PC1 is active on three substrates; lack of cleavage of pro- ANF and PAM in atrial myocytes is not due to a fundamental inability of atrial LDCV to support endoproteolytic processing.

Original languageEnglish (US)
Pages (from-to)5108-5118
Number of pages11
JournalEndocrinology
Volume138
Issue number12
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Adenovirally encoded prohormone convertase-1 functions in atrial myocyte large dense core vesicles'. Together they form a unique fingerprint.

  • Cite this