Adenoviral gene transfer of an NF-κB super-repressor increases collagen deposition in rodent cutaneous wound healing

Jeffrey Schreiber, Philip A. Efron, Julie E. Park, Lyle L. Moldawer, Adrian Barbul

Research output: Contribution to journalArticle

Abstract

Background. The transcription factor nuclear factor-κB (NF-κB) plays an essential role in inflammation. To date, no studies have investigated the effect of inhibiting NF-κB-mediated inflammation on normal cutaneous wound healing. We tested this by locally administering an adenovirus recombinant that constitutively expresses a super-repressor isoform of inhibitory-κB (IκB) into rats undergoing a well-established model of dorsal wound healing. Methods. Seventy-two Sprague-Dawley rats underwent insertion of a sponge-pump construct into a dorsal subcutaneous pocket. One group of rats received pumps filled with the adenovirus expressing I-κB (rAd-Iκb), a second group received pumps filled with adenovirus expressing green fluorescent protein (GFP) (rAd-gfp), and a third received pumps filled with normal saline (NS). Rats were killed in groups of 6 on days 1, 3, 5 and 7 postoperation. The wound fluid was analyzed for nitrite/nitrate (NOx) and tumor necrosis factor-alpha (TNF-α) concentrations. The wound fluid was assayed for hydroxyproline (OHP) content, an index of reparative collagen deposition. Results. Administration of rAd-Iκb for 7 days resulted in higher collagen deposition (OHP) compared with the rAd-gfp and NS groups. NOx levels were significantly higher in the rAd-gfp group on day 1 and marginally so on day 5. TNF-α quantitation analysis found no significant difference among the 3 groups. Conclusion. IκB expression through an adenoviral vector in the cutaneous wound may improve rodent healing, as shown by increased collagen deposition, through decreased inflammation. This mechanism appears to be TNF-α independent. Inhibition of NF-κB may reduce inflammation by reducing the local NOx concentrations.

Original languageEnglish (US)
Pages (from-to)940-946
Number of pages7
JournalSurgery
Volume138
Issue number5
DOIs
StatePublished - Nov 2005

Fingerprint

Transfer Factor
Wound Healing
Rodentia
Collagen
Adenoviridae
Inflammation
Skin
Tumor Necrosis Factor-alpha
Genes
Wounds and Injuries
Hydroxyproline
Porifera
Nitrites
Green Fluorescent Proteins
Nitrates
Sprague Dawley Rats
Protein Isoforms
Transcription Factors

ASJC Scopus subject areas

  • Surgery

Cite this

Adenoviral gene transfer of an NF-κB super-repressor increases collagen deposition in rodent cutaneous wound healing. / Schreiber, Jeffrey; Efron, Philip A.; Park, Julie E.; Moldawer, Lyle L.; Barbul, Adrian.

In: Surgery, Vol. 138, No. 5, 11.2005, p. 940-946.

Research output: Contribution to journalArticle

Schreiber, Jeffrey ; Efron, Philip A. ; Park, Julie E. ; Moldawer, Lyle L. ; Barbul, Adrian. / Adenoviral gene transfer of an NF-κB super-repressor increases collagen deposition in rodent cutaneous wound healing. In: Surgery. 2005 ; Vol. 138, No. 5. pp. 940-946.
@article{70ea77562c254abeb8e0a9975baee621,
title = "Adenoviral gene transfer of an NF-κB super-repressor increases collagen deposition in rodent cutaneous wound healing",
abstract = "Background. The transcription factor nuclear factor-κB (NF-κB) plays an essential role in inflammation. To date, no studies have investigated the effect of inhibiting NF-κB-mediated inflammation on normal cutaneous wound healing. We tested this by locally administering an adenovirus recombinant that constitutively expresses a super-repressor isoform of inhibitory-κB (IκB) into rats undergoing a well-established model of dorsal wound healing. Methods. Seventy-two Sprague-Dawley rats underwent insertion of a sponge-pump construct into a dorsal subcutaneous pocket. One group of rats received pumps filled with the adenovirus expressing I-κB (rAd-Iκb), a second group received pumps filled with adenovirus expressing green fluorescent protein (GFP) (rAd-gfp), and a third received pumps filled with normal saline (NS). Rats were killed in groups of 6 on days 1, 3, 5 and 7 postoperation. The wound fluid was analyzed for nitrite/nitrate (NOx) and tumor necrosis factor-alpha (TNF-α) concentrations. The wound fluid was assayed for hydroxyproline (OHP) content, an index of reparative collagen deposition. Results. Administration of rAd-Iκb for 7 days resulted in higher collagen deposition (OHP) compared with the rAd-gfp and NS groups. NOx levels were significantly higher in the rAd-gfp group on day 1 and marginally so on day 5. TNF-α quantitation analysis found no significant difference among the 3 groups. Conclusion. IκB expression through an adenoviral vector in the cutaneous wound may improve rodent healing, as shown by increased collagen deposition, through decreased inflammation. This mechanism appears to be TNF-α independent. Inhibition of NF-κB may reduce inflammation by reducing the local NOx concentrations.",
author = "Jeffrey Schreiber and Efron, {Philip A.} and Park, {Julie E.} and Moldawer, {Lyle L.} and Adrian Barbul",
year = "2005",
month = "11",
doi = "10.1016/j.surg.2005.05.020",
language = "English (US)",
volume = "138",
pages = "940--946",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Adenoviral gene transfer of an NF-κB super-repressor increases collagen deposition in rodent cutaneous wound healing

AU - Schreiber, Jeffrey

AU - Efron, Philip A.

AU - Park, Julie E.

AU - Moldawer, Lyle L.

AU - Barbul, Adrian

PY - 2005/11

Y1 - 2005/11

N2 - Background. The transcription factor nuclear factor-κB (NF-κB) plays an essential role in inflammation. To date, no studies have investigated the effect of inhibiting NF-κB-mediated inflammation on normal cutaneous wound healing. We tested this by locally administering an adenovirus recombinant that constitutively expresses a super-repressor isoform of inhibitory-κB (IκB) into rats undergoing a well-established model of dorsal wound healing. Methods. Seventy-two Sprague-Dawley rats underwent insertion of a sponge-pump construct into a dorsal subcutaneous pocket. One group of rats received pumps filled with the adenovirus expressing I-κB (rAd-Iκb), a second group received pumps filled with adenovirus expressing green fluorescent protein (GFP) (rAd-gfp), and a third received pumps filled with normal saline (NS). Rats were killed in groups of 6 on days 1, 3, 5 and 7 postoperation. The wound fluid was analyzed for nitrite/nitrate (NOx) and tumor necrosis factor-alpha (TNF-α) concentrations. The wound fluid was assayed for hydroxyproline (OHP) content, an index of reparative collagen deposition. Results. Administration of rAd-Iκb for 7 days resulted in higher collagen deposition (OHP) compared with the rAd-gfp and NS groups. NOx levels were significantly higher in the rAd-gfp group on day 1 and marginally so on day 5. TNF-α quantitation analysis found no significant difference among the 3 groups. Conclusion. IκB expression through an adenoviral vector in the cutaneous wound may improve rodent healing, as shown by increased collagen deposition, through decreased inflammation. This mechanism appears to be TNF-α independent. Inhibition of NF-κB may reduce inflammation by reducing the local NOx concentrations.

AB - Background. The transcription factor nuclear factor-κB (NF-κB) plays an essential role in inflammation. To date, no studies have investigated the effect of inhibiting NF-κB-mediated inflammation on normal cutaneous wound healing. We tested this by locally administering an adenovirus recombinant that constitutively expresses a super-repressor isoform of inhibitory-κB (IκB) into rats undergoing a well-established model of dorsal wound healing. Methods. Seventy-two Sprague-Dawley rats underwent insertion of a sponge-pump construct into a dorsal subcutaneous pocket. One group of rats received pumps filled with the adenovirus expressing I-κB (rAd-Iκb), a second group received pumps filled with adenovirus expressing green fluorescent protein (GFP) (rAd-gfp), and a third received pumps filled with normal saline (NS). Rats were killed in groups of 6 on days 1, 3, 5 and 7 postoperation. The wound fluid was analyzed for nitrite/nitrate (NOx) and tumor necrosis factor-alpha (TNF-α) concentrations. The wound fluid was assayed for hydroxyproline (OHP) content, an index of reparative collagen deposition. Results. Administration of rAd-Iκb for 7 days resulted in higher collagen deposition (OHP) compared with the rAd-gfp and NS groups. NOx levels were significantly higher in the rAd-gfp group on day 1 and marginally so on day 5. TNF-α quantitation analysis found no significant difference among the 3 groups. Conclusion. IκB expression through an adenoviral vector in the cutaneous wound may improve rodent healing, as shown by increased collagen deposition, through decreased inflammation. This mechanism appears to be TNF-α independent. Inhibition of NF-κB may reduce inflammation by reducing the local NOx concentrations.

UR - http://www.scopus.com/inward/record.url?scp=27744601774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744601774&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2005.05.020

DO - 10.1016/j.surg.2005.05.020

M3 - Article

VL - 138

SP - 940

EP - 946

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 5

ER -