Adenosine receptors on human leukocytes IV. characterization of an A1/Ri receptor

Gianni Marone; Rosaria Petracca; Sergio Vigorita; Arturo Genovese; Vincenzo Casolaro

doi:10.1007/BF02591429

Adenosine receptors on human leukocytes IV. characterization of an A1/Ri receptor

Gianni Marone, Rosaria Petracca, Sergio Vigorita, Arturo Genovese, Vincenzo Casolaro

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Adenosine (10^-9-10^-6 mol/l) and R-phenylisopropyladenosine (10^-9-10^-7 mol/l) partially inhibited the intracellular accumulation of cyclic AMP induced by isoproterenol, prostaglandin E₁, histamine and 5′-N-ethylcarboxamidoadenosine in lymphocytes. In contrast, S-phenylisopropyladenosine, which is a poor agonist of the adenosine A₁/R_i receptor, had essentially no inhibitory effect. 8-Phenyltheophylline, in low concentrations that do not inhibit cyclic AMP phosphodiesterase, completely blocked the inhibitory effect of R-phenylisopropyladenosine on the increase in cyclic AMP induced by prostaglandin E₁. R-Phenylisopropyladenosine (10^-8-10^-6 mol/l) also inhibited the cyclic AMP accumulation in lymphocytes induced by forskolin (10^-5 mol/l), which activates adenylate cyclase through direct interaction with the enzyme. We also investigated the presence of the adenosine A₁/R_i receptor on human polymorphonuclear leukocytes. R-Phenylisopropyladenosine (3×10^-9-10^-7 mol/l) abolished the stimulating effects of prostaglandin and forskolin on cyclic AMP accumulation in polymorphonuclear leukocytes. This effect was blocked by 8-phenyltheophylline and was not observed with the stereoisomer S-phenylisopropyladenosine. The results support the existence of an A₁/R_i receptor that regulates cyclic AMP metabolism of human lymphocytes and polymorphonuclear leukocytes.

Original language	English (US)
Pages (from-to)	235-242
Number of pages	8
Journal	International Journal of Clinical and Laboratory Research
Volume	22
Issue number	1-4
DOIs	https://doi.org/10.1007/BF02591429
State	Published - Mar 1992
Externally published	Yes

Keywords

A/R receptor
Adenosine
Cyclic AMP
Lymphocytes
Polymorphonuclear leukocytes

ASJC Scopus subject areas

Clinical Biochemistry

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10.1007/BF02591429

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title = "Adenosine receptors on human leukocytes IV. characterization of an A1/Ri receptor",

abstract = "Adenosine (10-9-10-6 mol/l) and R-phenylisopropyladenosine (10-9-10-7 mol/l) partially inhibited the intracellular accumulation of cyclic AMP induced by isoproterenol, prostaglandin E1, histamine and 5′-N-ethylcarboxamidoadenosine in lymphocytes. In contrast, S-phenylisopropyladenosine, which is a poor agonist of the adenosine A1/Ri receptor, had essentially no inhibitory effect. 8-Phenyltheophylline, in low concentrations that do not inhibit cyclic AMP phosphodiesterase, completely blocked the inhibitory effect of R-phenylisopropyladenosine on the increase in cyclic AMP induced by prostaglandin E1. R-Phenylisopropyladenosine (10-8-10-6 mol/l) also inhibited the cyclic AMP accumulation in lymphocytes induced by forskolin (10-5 mol/l), which activates adenylate cyclase through direct interaction with the enzyme. We also investigated the presence of the adenosine A1/Ri receptor on human polymorphonuclear leukocytes. R-Phenylisopropyladenosine (3×10-9-10-7 mol/l) abolished the stimulating effects of prostaglandin and forskolin on cyclic AMP accumulation in polymorphonuclear leukocytes. This effect was blocked by 8-phenyltheophylline and was not observed with the stereoisomer S-phenylisopropyladenosine. The results support the existence of an A1/Ri receptor that regulates cyclic AMP metabolism of human lymphocytes and polymorphonuclear leukocytes.",

keywords = "A/R receptor, Adenosine, Cyclic AMP, Lymphocytes, Polymorphonuclear leukocytes",

author = "Gianni Marone and Rosaria Petracca and Sergio Vigorita and Arturo Genovese and Vincenzo Casolaro",

year = "1992",

month = mar,

doi = "10.1007/BF02591429",

language = "English (US)",

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T1 - Adenosine receptors on human leukocytes IV. characterization of an A1/Ri receptor

AU - Marone, Gianni

AU - Petracca, Rosaria

AU - Vigorita, Sergio

AU - Genovese, Arturo

AU - Casolaro, Vincenzo

PY - 1992/3

Y1 - 1992/3

N2 - Adenosine (10-9-10-6 mol/l) and R-phenylisopropyladenosine (10-9-10-7 mol/l) partially inhibited the intracellular accumulation of cyclic AMP induced by isoproterenol, prostaglandin E1, histamine and 5′-N-ethylcarboxamidoadenosine in lymphocytes. In contrast, S-phenylisopropyladenosine, which is a poor agonist of the adenosine A1/Ri receptor, had essentially no inhibitory effect. 8-Phenyltheophylline, in low concentrations that do not inhibit cyclic AMP phosphodiesterase, completely blocked the inhibitory effect of R-phenylisopropyladenosine on the increase in cyclic AMP induced by prostaglandin E1. R-Phenylisopropyladenosine (10-8-10-6 mol/l) also inhibited the cyclic AMP accumulation in lymphocytes induced by forskolin (10-5 mol/l), which activates adenylate cyclase through direct interaction with the enzyme. We also investigated the presence of the adenosine A1/Ri receptor on human polymorphonuclear leukocytes. R-Phenylisopropyladenosine (3×10-9-10-7 mol/l) abolished the stimulating effects of prostaglandin and forskolin on cyclic AMP accumulation in polymorphonuclear leukocytes. This effect was blocked by 8-phenyltheophylline and was not observed with the stereoisomer S-phenylisopropyladenosine. The results support the existence of an A1/Ri receptor that regulates cyclic AMP metabolism of human lymphocytes and polymorphonuclear leukocytes.

AB - Adenosine (10-9-10-6 mol/l) and R-phenylisopropyladenosine (10-9-10-7 mol/l) partially inhibited the intracellular accumulation of cyclic AMP induced by isoproterenol, prostaglandin E1, histamine and 5′-N-ethylcarboxamidoadenosine in lymphocytes. In contrast, S-phenylisopropyladenosine, which is a poor agonist of the adenosine A1/Ri receptor, had essentially no inhibitory effect. 8-Phenyltheophylline, in low concentrations that do not inhibit cyclic AMP phosphodiesterase, completely blocked the inhibitory effect of R-phenylisopropyladenosine on the increase in cyclic AMP induced by prostaglandin E1. R-Phenylisopropyladenosine (10-8-10-6 mol/l) also inhibited the cyclic AMP accumulation in lymphocytes induced by forskolin (10-5 mol/l), which activates adenylate cyclase through direct interaction with the enzyme. We also investigated the presence of the adenosine A1/Ri receptor on human polymorphonuclear leukocytes. R-Phenylisopropyladenosine (3×10-9-10-7 mol/l) abolished the stimulating effects of prostaglandin and forskolin on cyclic AMP accumulation in polymorphonuclear leukocytes. This effect was blocked by 8-phenyltheophylline and was not observed with the stereoisomer S-phenylisopropyladenosine. The results support the existence of an A1/Ri receptor that regulates cyclic AMP metabolism of human lymphocytes and polymorphonuclear leukocytes.

KW - A/R receptor

KW - Adenosine

KW - Cyclic AMP

KW - Lymphocytes

KW - Polymorphonuclear leukocytes

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ER -

Adenosine receptors on human leukocytes IV. characterization of an A1/Ri receptor

Abstract

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