Adenosine 3′,5′-monophosphate waves in Dictyostelium discoideum: A demonstration by isotope dilution-fluorography

K. J. Tomchik, P. N. Devreotes

Research output: Contribution to journalArticle

Abstract

The distribution of adenosine 3′,5′-monophosphate (cyclic AMP), in fields of aggregating amoebae of Dictyostelium discoideum was examined by a nobel isotope dilution-fluorographic technique. Cellular cyclic AMP was visualized by its competition with exogenous 3H-labeled cyclic AMP for high-affinity binding sites on protein kinase immobilized on a Millipore filter used to blot the monolayer. The cyclic AMP was distributed in spiral or concentric circular wave patterns which centered on the foci of the aggregations. These patterns were correlated with those of cell shape change that propagate through the monolayers: cells in regions of high concentrations of cyclic AMP were elongated (presumably moving up a cyclic AMP gradient), whereas those in regions of low cyclic AMP concentrations were randomly directed. The highest cyclic AMP concentrations were about 10-6M. The widths of the regions of elevated cyclic AMP were about 0.3 to 1 millimeter which, assuming a wave velocity of 300 micrometers per minute, suggests that a cell signals for about 1 to 3 minutes. These observations support the hypothesis that the aggregation process in Dictyostelium 'is mediated by the periodic relay of cyclic AMP signals and suggest a simple scheme for the dynamics of the aggregation process.

Original languageEnglish (US)
Pages (from-to)443-445
Number of pages3
JournalScience
Volume212
Issue number4493
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • General

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