AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Yumi Jang; Fred Bunz

doi:10.1016/j.xpro.2022.101123

AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Yumi Jang, Fred Bunz

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).

Original language	English (US)
Article number	101123
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101123
State	Published - Mar 18 2022

Keywords

Biotechnology and bioengineering
Microbiology
Molecular Biology

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)
Medicine(all)

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10.1016/j.xpro.2022.101123

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abstract = "The AdenoBuilder platform enables the in vitro assembly of recombinant vectors from plasmid inserts that span the adenovirus genome. Two advantages of AdenoBuilder are the ease of modifying the genome and the ability to produce multicomponent vectors in a single step, facilitating parallel approaches to vector optimization. This protocol describes how to introduce transgenes in place of the endogenous Human Adenovirus serotype 5 (HAd5) E1 and/or E3 genes and can be applied to other parts of the HAd5 genome. For complete details on the use and execution of this protocol, please refer to Miciak et al. (2018).",

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AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses

Abstract

Keywords

ASJC Scopus subject areas

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