Additional diagnostic yield of adding serology to PCR in diagnosing viral acute respiratory infections in Kenyan patients 5 years of age and older

Daniel R. Feikin; M. Kariuki Njenga; Godfrey Bigogo; Barrack Aura; Stella Gikunju; Amanda Balish; Mark A. Katz; Dean Erdman; Robert F. Breiman

doi:10.1128/CVI.00325-12

Additional diagnostic yield of adding serology to PCR in diagnosing viral acute respiratory infections in Kenyan patients 5 years of age and older

Daniel R. Feikin, M. Kariuki Njenga, Godfrey Bigogo, Barrack Aura, Stella Gikunju, Amanda Balish, Mark A. Katz, Dean Erdman, Robert F. Breiman

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The role of serology in the setting of PCR-based diagnosis of acute respiratory infections (ARIs) is unclear. We found that acute- and convalescent-phase paired-sample serologic testing increased the diagnostic yield of naso/oropharyngeal swabs for influenza virus, respiratory syncytial virus (RSV), human metapneumovirus, adenovirus, and parainfluenza viruses beyond PCR by 0.4% to 10.7%. Although still limited for clinical use, serology, along with PCR, can maximize etiologic diagnosis in epidemiologic studies.

Original language	English (US)
Pages (from-to)	113-114
Number of pages	2
Journal	Clinical and Vaccine Immunology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1128/CVI.00325-12
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

Access to Document

10.1128/CVI.00325-12

Cite this

@article{974b38ed187743beafe1b36fcef2f3a8,

title = "Additional diagnostic yield of adding serology to PCR in diagnosing viral acute respiratory infections in Kenyan patients 5 years of age and older",

abstract = "The role of serology in the setting of PCR-based diagnosis of acute respiratory infections (ARIs) is unclear. We found that acute- and convalescent-phase paired-sample serologic testing increased the diagnostic yield of naso/oropharyngeal swabs for influenza virus, respiratory syncytial virus (RSV), human metapneumovirus, adenovirus, and parainfluenza viruses beyond PCR by 0.4% to 10.7%. Although still limited for clinical use, serology, along with PCR, can maximize etiologic diagnosis in epidemiologic studies.",

author = "Feikin, {Daniel R.} and Njenga, {M. Kariuki} and Godfrey Bigogo and Barrack Aura and Stella Gikunju and Amanda Balish and Katz, {Mark A.} and Dean Erdman and Breiman, {Robert F.}",

year = "2013",

month = jan,

doi = "10.1128/CVI.00325-12",

language = "English (US)",

volume = "20",

pages = "113--114",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "1",

}

TY - JOUR

T1 - Additional diagnostic yield of adding serology to PCR in diagnosing viral acute respiratory infections in Kenyan patients 5 years of age and older

AU - Feikin, Daniel R.

AU - Njenga, M. Kariuki

AU - Bigogo, Godfrey

AU - Aura, Barrack

AU - Gikunju, Stella

AU - Balish, Amanda

AU - Katz, Mark A.

AU - Erdman, Dean

AU - Breiman, Robert F.

PY - 2013/1

Y1 - 2013/1

N2 - The role of serology in the setting of PCR-based diagnosis of acute respiratory infections (ARIs) is unclear. We found that acute- and convalescent-phase paired-sample serologic testing increased the diagnostic yield of naso/oropharyngeal swabs for influenza virus, respiratory syncytial virus (RSV), human metapneumovirus, adenovirus, and parainfluenza viruses beyond PCR by 0.4% to 10.7%. Although still limited for clinical use, serology, along with PCR, can maximize etiologic diagnosis in epidemiologic studies.

AB - The role of serology in the setting of PCR-based diagnosis of acute respiratory infections (ARIs) is unclear. We found that acute- and convalescent-phase paired-sample serologic testing increased the diagnostic yield of naso/oropharyngeal swabs for influenza virus, respiratory syncytial virus (RSV), human metapneumovirus, adenovirus, and parainfluenza viruses beyond PCR by 0.4% to 10.7%. Although still limited for clinical use, serology, along with PCR, can maximize etiologic diagnosis in epidemiologic studies.

UR - http://www.scopus.com/inward/record.url?scp=84872232082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872232082&partnerID=8YFLogxK

U2 - 10.1128/CVI.00325-12

DO - 10.1128/CVI.00325-12

M3 - Article

C2 - 23114699

AN - SCOPUS:84872232082

SN - 1556-6811

VL - 20

SP - 113

EP - 114

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 1

ER -

Additional diagnostic yield of adding serology to PCR in diagnosing viral acute respiratory infections in Kenyan patients 5 years of age and older

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this