Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum

Fatma Simsek-Duran; David J. Linden; György Lonart

doi:10.1038/nn1348

Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum

Fatma Simsek-Duran, David J. Linden, György Lonart

School of Medicine

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1α at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1α, and that presynaptic transfection with a dominant-negative 14-3-3η mutant, or a RIM1α mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1α phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1α.

Original language	English (US)
Pages (from-to)	1296-1298
Number of pages	3
Journal	Nature neuroscience
Volume	7
Issue number	12
DOIs	https://doi.org/10.1038/nn1348
State	Published - Dec 2004

ASJC Scopus subject areas

General Neuroscience

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title = "Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum",

abstract = "Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1α at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1α, and that presynaptic transfection with a dominant-negative 14-3-3η mutant, or a RIM1α mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1α phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1α.",

author = "Fatma Simsek-Duran and Linden, {David J.} and Gy{\"o}rgy Lonart",

note = "Funding Information: We thank C. Howell, J. Mayer and S. Durkin for their help with the 14-3-3–RIM1α interaction studies and A.S. Shaw for providing 14-3-3 constructs. R. Bock provided technical assistance. Supported by an Eastern Virginia Medical School Bridge Grant, the Jeffress Memorial Trust (G.L.) and US Public Health Service MH51106 and the Develbiss Fund (D.J.L.). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Linden, David J.

AU - Lonart, György

N1 - Funding Information: We thank C. Howell, J. Mayer and S. Durkin for their help with the 14-3-3–RIM1α interaction studies and A.S. Shaw for providing 14-3-3 constructs. R. Bock provided technical assistance. Supported by an Eastern Virginia Medical School Bridge Grant, the Jeffress Memorial Trust (G.L.) and US Public Health Service MH51106 and the Develbiss Fund (D.J.L.). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/12

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N2 - Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1α at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1α, and that presynaptic transfection with a dominant-negative 14-3-3η mutant, or a RIM1α mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1α phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1α.

AB - Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1α at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1α, and that presynaptic transfection with a dominant-negative 14-3-3η mutant, or a RIM1α mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1α phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1α.

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Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum

Abstract

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