ADAM22 plays an important role in cell adhesion and spreading with the assistance of 14-3-3

Pengcheng Zhu, Yingying Sang, Huanbing Xu, Jing Zhao, Rener Xu, Yubo Sun, Tianhong Xu, Xiaolei Wang, Linfeng Chen, Hanping Feng, Changben Li, Shouyuan Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular adhesion plays important roles in a variety of biological processes. The ADAM family contains disintegrin-like and metalloproteinase-like domains which potentially have cell adhesion and protease activities. Recent studies suggest that the interaction between 14-3-3ζ and ADAM22cyt can regulate cell adhesion and spreading, therefore it has a potential role in neural development and function. 14-3-3 family has seven highly conserved members that regulate various cellular functions. Using yeast two-hybrid method, we identified that ADAM22cyt bound some other 14-3-3 family members. The interaction was further confirmed by in vitro protein pull-down assay and co-immunoprecipitation. We also found that the overexpression of exogenous ADAM22 in HEK293 cells could significantly enhance cell adhesion and spreading, compared with the truncated ADAM22 lack of 14-3-3 binding motifs. These results strongly demonstrated a functional role for ADAM22/14-3-3 in cell adhesion and spreading.

Original languageEnglish (US)
Pages (from-to)938-946
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
StatePublished - Jun 17 2005
Externally publishedYes

Keywords

  • 14-3-3
  • ADAM22
  • Cell adhesion
  • Cell spreading
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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