Acute renal failure produced by combining cyclosporine and brief renal ischemia in the Munich Wistar rat

B. C. Kone, Lorraine C Racusen, A. Whelton, K. Solez

Research output: Contribution to journalArticle

Abstract

To evaluate the combined effects of a brief ischemic insult and cyclosporine, four groups of male Munich Wistar rats were given: a) parenteral cyclosporine (60 mg/kg i.p.) for 4 days following 20 minutes of bilateral renal ischemia, b) the castor oil cyclosporine vehicle in a comparable volume and the same ischemic insult, c) saline in the same volume and ischemia, or d) saline and sham surgery. The cyclosporine animals ate and drank poorly, and therefore the other groups were pair-fed and watered with them. The cyclosporine-ischemia group developed significant renal failure. The other groups exhibited only a mild rise in blood urea nitrogen. Tubular vacuolization was a prominent feature in the cyclosporine and vehicle groups, but not in the saline group. Vacuolization was correlated with severity of renal impairment. Lipid stain showed that many of the vacuoles contained lipid. Eosinophilic cytoplasmic inclusions were seen only in the cyclosporine or vehicle- (castor oil) treated animals. These findings emphasize the probable functional importance of tubular lesions in cyclosporine-induced acute renal failure, and suggest that the castor oil vehicle of parenteral cyclosporine may have renal effects of its own.

Original languageEnglish (US)
JournalClinical Nephrology
Volume25
Issue numberSUPPL. 1
StatePublished - 1986

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Acute Kidney Injury
Cyclosporine
Wistar Rats
Ischemia
Kidney
Castor Oil
Lipids
Blood Urea Nitrogen
Inclusion Bodies
Vacuoles
Renal Insufficiency
Coloring Agents

ASJC Scopus subject areas

  • Nephrology

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Acute renal failure produced by combining cyclosporine and brief renal ischemia in the Munich Wistar rat. / Kone, B. C.; Racusen, Lorraine C; Whelton, A.; Solez, K.

In: Clinical Nephrology, Vol. 25, No. SUPPL. 1, 1986.

Research output: Contribution to journalArticle

Kone, B. C. ; Racusen, Lorraine C ; Whelton, A. ; Solez, K. / Acute renal failure produced by combining cyclosporine and brief renal ischemia in the Munich Wistar rat. In: Clinical Nephrology. 1986 ; Vol. 25, No. SUPPL. 1.
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