Acute pancreatitis in aging animals

Loss of pancreatitis-associated protein protection?

Sophia Fu, Albert Stanek, Cathy M. Mueller, Nefertti A. Brown, Chongmin Huan, Martin H. Bluth, Michael E. Zenilman

Research output: Contribution to journalArticle

Abstract

AIM: To investigate the effect of age on severity of acute pancreatitis (AP) using biochemical markers, histology and expression of the protective pancreatitis-associated proteins (PAPs). METHODS: AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats. Sera and pancreata were assayed at 24 h for the parameters listed above; we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA. RESULTS: At 24 h after induction of A P, the pancreata of older animals had less edema (mean ± SE histologic score of young vs old: 3.11 ± 0.16 vs 2.50 ± -0.11, P <0.05), decreased local inflammatory response (histologic score of stromal infiltrate: 3.11 ± 0.27 vs 2.00 ± 0.17, P <0.05) and increased bacterial infiltration (174% ± 52% increase from sham vs 377% ± 4%, P <0.05). A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohisto-chemical staining. There were no differences in serum amylase and lipase activity, or tissue myeloperoxidase or monocyte chemotactic protein-1 levels. However, in the most-aged group, serum C-reactive protein levels were higher (young vs old: 0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL, P <0.05). CONCLUSION: In older animals, there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level; this may explain the more aggressive clinical course.

Original languageEnglish (US)
Pages (from-to)3379-3388
Number of pages10
JournalWorld Journal of Gastroenterology
Volume18
Issue number26
DOIs
StatePublished - Jul 2012

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Pancreatitis
C-Reactive Protein
Pancreas
Taurocholic Acid
Ribosomal RNA
Chemokine CCL2
Amylases
Serum
Lipase
Peroxidase
Blood Proteins
Edema
Histology
Biomarkers
Western Blotting
Staining and Labeling
Polymerase Chain Reaction
Injections
pancreatitis-associated protein

Keywords

  • Acute pancreatitis
  • Aging
  • Molecular biology
  • Pancreatitis-associated protein
  • Rats

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Fu, S., Stanek, A., Mueller, C. M., Brown, N. A., Huan, C., Bluth, M. H., & Zenilman, M. E. (2012). Acute pancreatitis in aging animals: Loss of pancreatitis-associated protein protection? World Journal of Gastroenterology, 18(26), 3379-3388. https://doi.org/10.3748/wjg.v18.i26.3379

Acute pancreatitis in aging animals : Loss of pancreatitis-associated protein protection? / Fu, Sophia; Stanek, Albert; Mueller, Cathy M.; Brown, Nefertti A.; Huan, Chongmin; Bluth, Martin H.; Zenilman, Michael E.

In: World Journal of Gastroenterology, Vol. 18, No. 26, 07.2012, p. 3379-3388.

Research output: Contribution to journalArticle

Fu, S, Stanek, A, Mueller, CM, Brown, NA, Huan, C, Bluth, MH & Zenilman, ME 2012, 'Acute pancreatitis in aging animals: Loss of pancreatitis-associated protein protection?', World Journal of Gastroenterology, vol. 18, no. 26, pp. 3379-3388. https://doi.org/10.3748/wjg.v18.i26.3379
Fu, Sophia ; Stanek, Albert ; Mueller, Cathy M. ; Brown, Nefertti A. ; Huan, Chongmin ; Bluth, Martin H. ; Zenilman, Michael E. / Acute pancreatitis in aging animals : Loss of pancreatitis-associated protein protection?. In: World Journal of Gastroenterology. 2012 ; Vol. 18, No. 26. pp. 3379-3388.
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abstract = "AIM: To investigate the effect of age on severity of acute pancreatitis (AP) using biochemical markers, histology and expression of the protective pancreatitis-associated proteins (PAPs). METHODS: AP was induced via intraductal injection of 4{\%} sodium taurocholate in young and old rats. Sera and pancreata were assayed at 24 h for the parameters listed above; we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA. RESULTS: At 24 h after induction of A P, the pancreata of older animals had less edema (mean ± SE histologic score of young vs old: 3.11 ± 0.16 vs 2.50 ± -0.11, P <0.05), decreased local inflammatory response (histologic score of stromal infiltrate: 3.11 ± 0.27 vs 2.00 ± 0.17, P <0.05) and increased bacterial infiltration (174{\%} ± 52{\%} increase from sham vs 377{\%} ± 4{\%}, P <0.05). A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohisto-chemical staining. There were no differences in serum amylase and lipase activity, or tissue myeloperoxidase or monocyte chemotactic protein-1 levels. However, in the most-aged group, serum C-reactive protein levels were higher (young vs old: 0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL, P <0.05). CONCLUSION: In older animals, there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level; this may explain the more aggressive clinical course.",
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AU - Bluth, Martin H.

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