Acute hepatitis C virus infection induces consistent changes in circulating MicroRNAs that are associated with nonlytic hepatocyte release

Ramy El-Diwany, Lisa N. Wasilewski, Kenneth W. Witwer, Justin R. Bailey, Kimberly Page, Stuart C. Ray, Andrea L. Cox, David L. Thomas, Ashwin Balagopal

Research output: Contribution to journalArticle

Abstract

Plasma microRNAs (miRNAs) change in abundance in response to disease and have been associated with liver fibrosis severity in chronic hepatitis C virus (HCV) infection. However, the early dynamics of miRNA release during acute HCV infection are poorly understood. In addition, circulating miRNA signatures have been difficult to reproduce among separate populations. We studied plasma miRNA abundance during acute HCV infection to identify an miRNA signature of early infection. We measured 754 plasma miRNAs by quantitative PCR array in a discovery cohort of 22 individuals before and during acute HCV infection and after spontaneous resolution (n = 11) or persistence (n = 11) to identify a plasma miRNA signature. The discovery cohort derived from the Baltimore Before and After Acute Study of Hepatitis. During acute HCV infection, increases in miR-122 (P<0.01) and miR-885-5p (Pcorrectedcorrectedp despite unperturbed intracellular levels. In contrast, miR-494 accumulated intracellularly (P

Original languageEnglish (US)
Pages (from-to)9454-9464
Number of pages11
JournalJournal of Virology
Volume89
Issue number18
DOIs
StatePublished - 2015

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MicroRNAs
Hepacivirus
Hepatocytes
Infection
microRNA
infection
Hepatitis C virus
Baltimore
Chronic Hepatitis C
Liver Cirrhosis
Hepatitis
Polymerase Chain Reaction
chronic hepatitis C
liver cirrhosis
hepatitis
hepatocytes
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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title = "Acute hepatitis C virus infection induces consistent changes in circulating MicroRNAs that are associated with nonlytic hepatocyte release",
abstract = "Plasma microRNAs (miRNAs) change in abundance in response to disease and have been associated with liver fibrosis severity in chronic hepatitis C virus (HCV) infection. However, the early dynamics of miRNA release during acute HCV infection are poorly understood. In addition, circulating miRNA signatures have been difficult to reproduce among separate populations. We studied plasma miRNA abundance during acute HCV infection to identify an miRNA signature of early infection. We measured 754 plasma miRNAs by quantitative PCR array in a discovery cohort of 22 individuals before and during acute HCV infection and after spontaneous resolution (n = 11) or persistence (n = 11) to identify a plasma miRNA signature. The discovery cohort derived from the Baltimore Before and After Acute Study of Hepatitis. During acute HCV infection, increases in miR-122 (P<0.01) and miR-885-5p (Pcorrectedcorrectedp despite unperturbed intracellular levels. In contrast, miR-494 accumulated intracellularly (P",
author = "Ramy El-Diwany and Wasilewski, {Lisa N.} and Witwer, {Kenneth W.} and Bailey, {Justin R.} and Kimberly Page and Ray, {Stuart C.} and Cox, {Andrea L.} and Thomas, {David L.} and Ashwin Balagopal",
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AU - El-Diwany,Ramy

AU - Wasilewski,Lisa N.

AU - Witwer,Kenneth W.

AU - Bailey,Justin R.

AU - Page,Kimberly

AU - Ray,Stuart C.

AU - Cox,Andrea L.

AU - Thomas,David L.

AU - Balagopal,Ashwin

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AB - Plasma microRNAs (miRNAs) change in abundance in response to disease and have been associated with liver fibrosis severity in chronic hepatitis C virus (HCV) infection. However, the early dynamics of miRNA release during acute HCV infection are poorly understood. In addition, circulating miRNA signatures have been difficult to reproduce among separate populations. We studied plasma miRNA abundance during acute HCV infection to identify an miRNA signature of early infection. We measured 754 plasma miRNAs by quantitative PCR array in a discovery cohort of 22 individuals before and during acute HCV infection and after spontaneous resolution (n = 11) or persistence (n = 11) to identify a plasma miRNA signature. The discovery cohort derived from the Baltimore Before and After Acute Study of Hepatitis. During acute HCV infection, increases in miR-122 (P<0.01) and miR-885-5p (Pcorrectedcorrectedp despite unperturbed intracellular levels. In contrast, miR-494 accumulated intracellularly (P

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