Acute exposure of apigenin induces hepatotoxicity in Swiss mice

Prabhat Singh, Shrawan Kumar Mishra, Sanjeev Noel, Sharad Sharma, Srikanta Kumar Rath

Research output: Contribution to journalArticle

Abstract

Apigenin, a dietary flavonoid, is reported to have several therapeutic effects in different diseases including cancer. Toxicity of Apigenin is however, least explored, and reports are scanty in literature. This warrants dose-specific evaluation of toxicity in vivo. In the present study, Apigenin was administered intraperitoneally to Swiss mice at doses of 25, 50, 100 and 200 mg/kg. Serum levels of alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP) were measured along with the examination of liver histology, reactive oxygen species (ROS) in blood, lipid peroxidation (LPO), glutathione level, superoxide dismutase activity, catalase activity, glutathione S-transferase activity and gene expression in liver tissue. Increase in ALT, AST, ALP, ROS, ratio of oxidized to reduced glutathione (GSSG/GSH) and LPO, altered enzyme activities along with damaged histoarchitecture in the liver of 100 or 200 mg/kg Apigenin treated animals were found. Microarray analysis revealed the differential expression of genes that correspond to different biologically relevant pathways including oxidative stress and apoptosis. In conclusion, these results suggested the oxidative stress induced liver damage which may be due to the regulation of multiple genes by Apigenin at higher doses in Swiss mice.

Original languageEnglish (US)
Article numbere31964
JournalPLoS One
Volume7
Issue number2
DOIs
StatePublished - Feb 16 2012
Externally publishedYes

Fingerprint

Apigenin
acute exposure
hepatotoxicity
apigenin
Transferases
Liver
mice
liver
Oxidative stress
alanine transaminase
Aspartic Acid
aspartate transaminase
Alanine
Lipid Peroxidation
Glutathione
Alkaline Phosphatase
alkaline phosphatase
Toxicity
glutathione
reactive oxygen species

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Acute exposure of apigenin induces hepatotoxicity in Swiss mice. / Singh, Prabhat; Mishra, Shrawan Kumar; Noel, Sanjeev; Sharma, Sharad; Rath, Srikanta Kumar.

In: PLoS One, Vol. 7, No. 2, e31964, 16.02.2012.

Research output: Contribution to journalArticle

Singh, Prabhat ; Mishra, Shrawan Kumar ; Noel, Sanjeev ; Sharma, Sharad ; Rath, Srikanta Kumar. / Acute exposure of apigenin induces hepatotoxicity in Swiss mice. In: PLoS One. 2012 ; Vol. 7, No. 2.
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