Activity-dependent secretion of neuronal activity regulated pentraxin from vasopressin neurons into the systemic circulation

I. M. Reti, M. Miskimon, M. Dickson, R. S. Petralia, K. Takamiya, R. Bland, J. Saini, M. J. During, R. L. Huganir, J. M. Baraban

Research output: Contribution to journalArticlepeer-review


Neuronal activity regulated pentraxin (Narp) is a secreted, synaptic protein that has been implicated in modulating synaptic transmission. However, it is unclear how Narp secretion is regulated. Since we noted prominent Narp immunostaining in vasopressin neurons of the hypothalamus and in the posterior pituitary, we assessed whether it, like vasopressin, is released into the systemic circulation in an activity-dependent fashion. Consistent with this hypothesis, electron microscopic studies of the posterior pituitary demonstrated that Narp is located in secretory vesicles containing vasopressin. Using affinity chromatography, we detected Narp in plasma and found that these levels are markedly decreased by hypophysectomy. In addition, we confirmed that injection of a viral Narp construct into the hypothalamus restores plasma Narp levels in Narp knockout mice. In checking for activity-dependent secretion of Narp from the posterior pituitary, we found that several stimuli known to trigger vasopressin release, i.e. hypovolemia, dehydration and endotoxin, elevate plasma Narp levels. Taken together, these findings provide compelling evidence that Narp is secreted from vasopressin neurons in an activity-dependent fashion.

Original languageEnglish (US)
Pages (from-to)352-360
Number of pages9
Issue number2
StatePublished - Jan 24 2008


  • dehydration
  • hypophysectomy
  • hypothalamus
  • hypovolemia
  • pituitary
  • plasma

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Activity-dependent secretion of neuronal activity regulated pentraxin from vasopressin neurons into the systemic circulation'. Together they form a unique fingerprint.

Cite this