TY - JOUR
T1 - Activities of WIN-57273, minocycline, clarithromycin, and 14-hydroxy- clarithromycin against Mycobacterium avium complex in human macrophages
AU - Cohen, Y.
AU - Perronne, C.
AU - Truffot-Pernot, C.
AU - Grosset, J.
AU - Vilde, J. L.
AU - Pocidalo -, J. J.
PY - 1992
Y1 - 1992
N2 - The activities of the fluoroquinolone WIN-57273, 14-OH clarithromycin (a human metabolite of clarithromycin), and minocycline against two virulent strains of Mycobacterium avium complex were evaluated in a model of intracellular infection and compared with that of clarithromycin. Human monocyte-derived macrophages were infected at day 6 of culture. Intracellular CFU at 60 min and intracellular and supernatant CFU on days 4 and 7 were counted after inoculation. The concentrations used, which were equal to peak levels in serum, were 3 μg of WIN-57273 per ml (MICs for the two strains, 1 μg/ml), 4 μg of 14-OH clarithromycin per ml (MICs, 8 and 2 μg/ml, respectively, at pH 7.4), 4 μg of minocycline per ml (MICs, 64 and 32 μg/ml, respectively), and 4 μg of clarithromycin per ml (MICs, 2 and 0.5 μg/ml, respectively, at pH 7.4). On day 7, compared with controls, WIN- 57273, minocycline (P < 0.02), clarithromycin, or different combinations of clarithromycin and the other drugs (P < 0.001) slowed the intracellular replication of strain MO-1. 14-OH clarithromycin (P < 0.02), clarithromycin (P < 0.02), 14-OH clarithromycin plus clarithromycin (P < 0.01), clarithromycin plus minocycline, or clarithromycin plus minocycline plus 14- OH clarithromycin (P < 0.001) slowed the intracellular replication of strain LV-2. WIN-57273 was less effective than clarithromycin against strain MO-1 (P < 0.05). Clarithromycin plus 14-OH clarithromycin plus minocycline (P < 0.02) was more effective than clarithromycin alone against strain LV-2. Thus, clarithromycin plus minocycline, which corresponds in humans to three active molecules, may exhibit a better efficacy than clarithromycin in this model.
AB - The activities of the fluoroquinolone WIN-57273, 14-OH clarithromycin (a human metabolite of clarithromycin), and minocycline against two virulent strains of Mycobacterium avium complex were evaluated in a model of intracellular infection and compared with that of clarithromycin. Human monocyte-derived macrophages were infected at day 6 of culture. Intracellular CFU at 60 min and intracellular and supernatant CFU on days 4 and 7 were counted after inoculation. The concentrations used, which were equal to peak levels in serum, were 3 μg of WIN-57273 per ml (MICs for the two strains, 1 μg/ml), 4 μg of 14-OH clarithromycin per ml (MICs, 8 and 2 μg/ml, respectively, at pH 7.4), 4 μg of minocycline per ml (MICs, 64 and 32 μg/ml, respectively), and 4 μg of clarithromycin per ml (MICs, 2 and 0.5 μg/ml, respectively, at pH 7.4). On day 7, compared with controls, WIN- 57273, minocycline (P < 0.02), clarithromycin, or different combinations of clarithromycin and the other drugs (P < 0.001) slowed the intracellular replication of strain MO-1. 14-OH clarithromycin (P < 0.02), clarithromycin (P < 0.02), 14-OH clarithromycin plus clarithromycin (P < 0.01), clarithromycin plus minocycline, or clarithromycin plus minocycline plus 14- OH clarithromycin (P < 0.001) slowed the intracellular replication of strain LV-2. WIN-57273 was less effective than clarithromycin against strain MO-1 (P < 0.05). Clarithromycin plus 14-OH clarithromycin plus minocycline (P < 0.02) was more effective than clarithromycin alone against strain LV-2. Thus, clarithromycin plus minocycline, which corresponds in humans to three active molecules, may exhibit a better efficacy than clarithromycin in this model.
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U2 - 10.1128/AAC.36.10.2104
DO - 10.1128/AAC.36.10.2104
M3 - Article
C2 - 1332586
AN - SCOPUS:0026641417
VL - 36
SP - 2104
EP - 2107
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 10
ER -