Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells

Sun Eun Lee, Joo Weon Lim, Hyeyoung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Docosahexaenoic acid (DHA) shows anti-inflammatory and/or anticancer effects in some cells. Activator protein-1 (AP-1) regulates cellular proliferation and apoptosis. Although recent studies demonstrate the association between gastric cancer risk and DHA, the exact molecular mechanism has not been clarified. We investigated whether AP-1 mediates DHA-induced apoptosis of gastric cancer cells. We found that DHA induced cell death and DNA fragmentation in parallel with the activation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) as well as AP-1. DHA increased the protein levels of p53, cytochrome c, and Bax in gastric cancer cells. DHA-induced DNA fragmentation and protein levels of p53, cytochrome c, and Bax were inhibited in the cells transfected with c-jun dominant-negative mutant (TAM67). Because JNK and ERK are upstream signaling for AP-1 activation, we suggest that DHA-induced activation of AP-1 may mediate apoptosis of gastric cancer cells by inducing the expression of apoptotic genes in gastric cancer cells.

Original languageEnglish (US)
Title of host publicationNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
Pages163-169
Number of pages7
Volume1171
DOIs
StatePublished - Aug 2009
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Docosahexaenoic Acids
Transcription Factor AP-1
Stomach Neoplasms
Cells
Apoptosis
Chemical activation
Extracellular Signal-Regulated MAP Kinases
DNA Fragmentation
Cytochromes c
JNK Mitogen-Activated Protein Kinases
DNA
Cell death
Proteins
Cell Death
Anti-Inflammatory Agents
Phosphotransferases
Genes
Cell Proliferation
Association reactions
Gene Expression

Keywords

  • AP-1
  • Apoptosis
  • DHA
  • Gastric cancer cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lee, S. E., Lim, J. W., & Kim, H. (2009). Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (Vol. 1171, pp. 163-169). (Annals of the New York Academy of Sciences; Vol. 1171). https://doi.org/10.1111/j.1749-6632.2009.04716.x

Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. / Lee, Sun Eun; Lim, Joo Weon; Kim, Hyeyoung.

Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Vol. 1171 2009. p. 163-169 (Annals of the New York Academy of Sciences; Vol. 1171).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lee, SE, Lim, JW & Kim, H 2009, Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. in Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. vol. 1171, Annals of the New York Academy of Sciences, vol. 1171, pp. 163-169. https://doi.org/10.1111/j.1749-6632.2009.04716.x
Lee SE, Lim JW, Kim H. Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Vol. 1171. 2009. p. 163-169. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04716.x
Lee, Sun Eun ; Lim, Joo Weon ; Kim, Hyeyoung. / Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Vol. 1171 2009. pp. 163-169 (Annals of the New York Academy of Sciences).
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