Activation of the STAT pathway in acute lung injury

Mariano Severgnini, Satoe Takahashi, Liliana M. Rozo, Robert J. Homer, Charles Kuhn, Jhung W. Jhung, George Perides, Michael Steer, Paul M Hassoun, Barry L. Fanburg, Brent H. Cochran, Amy R. Simon

Research output: Contribution to journalArticle

Abstract

Acute lung injury (ALI) is a devastating clinical problem with a mortality as high as 60%. It is now appreciated that ALI represents a cytokine excess state that involves the microvasculature of multiple organs. The signal transducers and activators of transcription (STAT) family of transcription factors activate critical mediators of cytokine responses, but there is limited knowledge about their role in mediating ALI. In the present study, we demonstrate that the STAT transcription factors are activated rapidly in the lungs after intraperitoneal and intranasal LPS administration in mice. We also demonstrated that LPS activates both the STAT kinases, Src and JAK, in the lung with kinetics that are consistent with STAT activation. LPS treatment resulted in STAT3 activation throughout the resident lung cells, as well as in the recruited inflammatory cells. Whereas direct LPS treatment did not lead to STAT activation in cultured epithelial or endothelial cells, IL-6 activated STAT3 in both of these cell types. Furthermore, IL-6 was induced by LPS in serum and in the lung with kinetics consistent with STAT3 activation, suggesting that IL-6 may be one mechanism of STAT activation by LPS. In addition, STAT activation required reactive oxygen species, as the overexpression of catalase in mice prevented LPS-mediated STAT activation in the lung. STATs may be a common pathway for mediating ALI, regardless of the inciting factor, as STAT activation also occurred in both a gastric acid aspiration and acute pancreatitis model of ALI. Finally, STATs are activated in the lung long before signs of ALI are present, suggesting that the STAT transcription factors may play a role in initiating the inflammatory response seen in the lung.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume286
Issue number6 30-6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Acute Lung Injury
Transducers
Transcriptional Activation
Lung
Interleukin-6
Transcription Factors
Cytokines
Janus Kinases
Intranasal Administration
src-Family Kinases
Gastric Acid
Microvessels
Pancreatitis
Catalase
Cultured Cells
Reactive Oxygen Species
Endothelial Cells
Epithelial Cells
Mortality

Keywords

  • Lipopolysaccharide
  • Reactive oxygen species
  • Signal transducers and activators of transcription

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Severgnini, M., Takahashi, S., Rozo, L. M., Homer, R. J., Kuhn, C., Jhung, J. W., ... Simon, A. R. (2004). Activation of the STAT pathway in acute lung injury. American Journal of Physiology - Lung Cellular and Molecular Physiology, 286(6 30-6). https://doi.org/10.1152/ajplung.00349.2003

Activation of the STAT pathway in acute lung injury. / Severgnini, Mariano; Takahashi, Satoe; Rozo, Liliana M.; Homer, Robert J.; Kuhn, Charles; Jhung, Jhung W.; Perides, George; Steer, Michael; Hassoun, Paul M; Fanburg, Barry L.; Cochran, Brent H.; Simon, Amy R.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 286, No. 6 30-6, 06.2004.

Research output: Contribution to journalArticle

Severgnini, M, Takahashi, S, Rozo, LM, Homer, RJ, Kuhn, C, Jhung, JW, Perides, G, Steer, M, Hassoun, PM, Fanburg, BL, Cochran, BH & Simon, AR 2004, 'Activation of the STAT pathway in acute lung injury', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 286, no. 6 30-6. https://doi.org/10.1152/ajplung.00349.2003
Severgnini, Mariano ; Takahashi, Satoe ; Rozo, Liliana M. ; Homer, Robert J. ; Kuhn, Charles ; Jhung, Jhung W. ; Perides, George ; Steer, Michael ; Hassoun, Paul M ; Fanburg, Barry L. ; Cochran, Brent H. ; Simon, Amy R. / Activation of the STAT pathway in acute lung injury. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; Vol. 286, No. 6 30-6.
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AU - Jhung, Jhung W.

AU - Perides, George

AU - Steer, Michael

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