TY - JOUR
T1 - Activation of the endoplasmic reticulum stress response in autoimmune myositis
T2 - Potential role in muscle fiber damage and dysfunction
AU - Nagaraju, Kanneboyina
AU - Casciola-Rosen, Livia
AU - Lundberg, Ingrid
AU - Rawat, Rashmi
AU - Cutting, Shawna
AU - Thapliyal, Rachana
AU - Chang, Jason
AU - Dwivedi, Sunita
AU - Mitsak, Megan
AU - Chen, Yi Wen
AU - Plotz, Paul
AU - Rosen, Antony
AU - Hoffman, Eric
AU - Raben, Nina
PY - 2005/6
Y1 - 2005/6
N2 - Objective. The etiology and pathogenesis of human inflammatory myopathies remain unclear. Findings of several studies suggest that the degree of inflammation does not correlate consistently with the severity of clinical disease or of structural changes in the muscle fibers, indicating that nonimmune pathways may contribute to the pathogenesis of myositis. This study was undertaken to investigate these pathways in myositis patients and in a class I major histocompatibility complex (MHC)-transgenic mouse model of myositis. Methods. We examined muscle tissue from human myositis patients and from class I MHC-transgenic mice for nonimmune pathways, using biochemical, immunohistochemical, and gene expression profiling assays. Results. Up-regulation of class I MHC in skeletal muscle fibers was an early and consistent feature of human inflammatory myopathies. Class I MHC staining in muscle fibers of myositis patients showed both cell surface and a reticular pattern of internal reactivity. The pathways of endoplasmic reticulum (ER) stress response, the unfolded protein response (glucose-regulated protein 78 pathway), and the ER overload response (NF-κB pathway) were significantly activated in muscle tissue of human myositis patients and in the mouse model. Ectopic expression of wild-type mouse class I MHC (H-2Kb) but not degradable glycosylation mutants of H-2Kb induced ER stress response in C2C12 skeletal muscle cells. Conclusion. These results indicate that the ER stress response may be a major nonimmune mechanism responsible for skeletal muscle damage and dysfunction in autoimmune myositis. Strategies to interfere with this pathway may have therapeutic value in patients with this disease.
AB - Objective. The etiology and pathogenesis of human inflammatory myopathies remain unclear. Findings of several studies suggest that the degree of inflammation does not correlate consistently with the severity of clinical disease or of structural changes in the muscle fibers, indicating that nonimmune pathways may contribute to the pathogenesis of myositis. This study was undertaken to investigate these pathways in myositis patients and in a class I major histocompatibility complex (MHC)-transgenic mouse model of myositis. Methods. We examined muscle tissue from human myositis patients and from class I MHC-transgenic mice for nonimmune pathways, using biochemical, immunohistochemical, and gene expression profiling assays. Results. Up-regulation of class I MHC in skeletal muscle fibers was an early and consistent feature of human inflammatory myopathies. Class I MHC staining in muscle fibers of myositis patients showed both cell surface and a reticular pattern of internal reactivity. The pathways of endoplasmic reticulum (ER) stress response, the unfolded protein response (glucose-regulated protein 78 pathway), and the ER overload response (NF-κB pathway) were significantly activated in muscle tissue of human myositis patients and in the mouse model. Ectopic expression of wild-type mouse class I MHC (H-2Kb) but not degradable glycosylation mutants of H-2Kb induced ER stress response in C2C12 skeletal muscle cells. Conclusion. These results indicate that the ER stress response may be a major nonimmune mechanism responsible for skeletal muscle damage and dysfunction in autoimmune myositis. Strategies to interfere with this pathway may have therapeutic value in patients with this disease.
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U2 - 10.1002/art.21103
DO - 10.1002/art.21103
M3 - Article
C2 - 15934115
AN - SCOPUS:20744448833
SN - 0004-3591
VL - 52
SP - 1824
EP - 1835
JO - Arthritis and rheumatism
JF - Arthritis and rheumatism
IS - 6
ER -