Activation of retinoid X receptors induces apoptosis in HL-60 cell lines

Laszlo Nagy, Vilmos A. Thomázy, Gregory L. Shipley, László Fésüs, William Lamph, Richard A. Heyman, Roshantha A.S. Chandraratna, Peter J.A. Davies

Research output: Contribution to journalArticle

Abstract

Retinoids induce myeloblastic leukemia (HL-60) cells to differentiate into granulocytes, which subsequently die by apoptosis. Retinoid action is mediated through at least two classes of nuclear receptors: retinoic acid receptors, which bind both all-trans retinoic acid and 9-cis retinoic acid, and retinoid X receptors, which bind only 9-cis retinoic acid. Using receptor-selective synthetic retinoids and HL-60 cell sublines with different retinoid responsiveness, we have investigated the contribution that each class of receptors makes to the processes of cellular differentiation and death. Our results demonstrate that ligand activation of retinoic acid receptors is sufficient to induce differentiation, whereas ligand activation of retinoid X receptors is essential for the induction of apoptosis in HL-60 cell lines.

Original languageEnglish (US)
Pages (from-to)3540-3551
Number of pages12
JournalMolecular and Cellular Biology
Volume15
Issue number7
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Retinoid X Receptors
HL-60 Cells
Retinoids
Apoptosis
Cell Line
Retinoic Acid Receptors
Artificial Receptors
Ligands
Cytoplasmic and Nuclear Receptors
Tretinoin
Granulocytes
Leukemia
alitretinoin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Nagy, L., Thomázy, V. A., Shipley, G. L., Fésüs, L., Lamph, W., Heyman, R. A., ... Davies, P. J. A. (1995). Activation of retinoid X receptors induces apoptosis in HL-60 cell lines. Molecular and Cellular Biology, 15(7), 3540-3551. https://doi.org/10.1128/MCB.15.7.3540

Activation of retinoid X receptors induces apoptosis in HL-60 cell lines. / Nagy, Laszlo; Thomázy, Vilmos A.; Shipley, Gregory L.; Fésüs, László; Lamph, William; Heyman, Richard A.; Chandraratna, Roshantha A.S.; Davies, Peter J.A.

In: Molecular and Cellular Biology, Vol. 15, No. 7, 01.01.1995, p. 3540-3551.

Research output: Contribution to journalArticle

Nagy, L, Thomázy, VA, Shipley, GL, Fésüs, L, Lamph, W, Heyman, RA, Chandraratna, RAS & Davies, PJA 1995, 'Activation of retinoid X receptors induces apoptosis in HL-60 cell lines', Molecular and Cellular Biology, vol. 15, no. 7, pp. 3540-3551. https://doi.org/10.1128/MCB.15.7.3540
Nagy, Laszlo ; Thomázy, Vilmos A. ; Shipley, Gregory L. ; Fésüs, László ; Lamph, William ; Heyman, Richard A. ; Chandraratna, Roshantha A.S. ; Davies, Peter J.A. / Activation of retinoid X receptors induces apoptosis in HL-60 cell lines. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 7. pp. 3540-3551.
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