TY - JOUR
T1 - Activation of programmed cell death by recombinant human tumor necrosis factor plus topoisomerase II - targeted drugs in L929 tumor cells
AU - Kyprianou, Natasha
AU - Alexander, Richard B.
AU - Isaacs, John T.
N1 - Funding Information:
Supported by Public Health Service grant CA-50601 from the National Cancer Institute, National Institutes of Health, Department of Health and Human Services.
PY - 1991/3/6
Y1 - 1991/3/6
N2 - The mechanism of death induced by recombinant human tumor necrosis factor (rHuTNF) in L929 tumor cells of C3H mice was investigated. Treatment with rHuTNF led to fragmentation of DNA into nucleosomal oligomers and to induction of the expression of TRPM-2, a programmed cell death-associated gene. Both events preceded cell death by several hours. Treatment with DNA topoisomerase II inhibitors accelerated both the rHuTNF-mediated DNA fragmentation and the elevation in TRPM-2 messenger RNA levels. These results suggest that rHuTNF exerts its cytotoxicity on L929 cells by activating programmed cell death, leading to apoptosis, and that topoisomerase II inhibitors enhance rHuTNF-mediated cytotoxicity by accelerating this process. [J Natl Cancer Inst 83:346-350, 1991].
AB - The mechanism of death induced by recombinant human tumor necrosis factor (rHuTNF) in L929 tumor cells of C3H mice was investigated. Treatment with rHuTNF led to fragmentation of DNA into nucleosomal oligomers and to induction of the expression of TRPM-2, a programmed cell death-associated gene. Both events preceded cell death by several hours. Treatment with DNA topoisomerase II inhibitors accelerated both the rHuTNF-mediated DNA fragmentation and the elevation in TRPM-2 messenger RNA levels. These results suggest that rHuTNF exerts its cytotoxicity on L929 cells by activating programmed cell death, leading to apoptosis, and that topoisomerase II inhibitors enhance rHuTNF-mediated cytotoxicity by accelerating this process. [J Natl Cancer Inst 83:346-350, 1991].
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U2 - 10.1093/jnci/83.5.346
DO - 10.1093/jnci/83.5.346
M3 - Article
C2 - 1847434
AN - SCOPUS:0026417742
SN - 0027-8874
VL - 83
SP - 346
EP - 350
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -